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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008-10-27 to 2008-11-06
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not applicable
Principles of method if other than guideline:
not concerned
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): LCE07105
- Substance type: solid, white flakes
- Physical state: solid
- Analytical purity: >99%
- Impurities (identity and concentrations): no ipurities identified
- Composition of test material, percentage of components: Alcohol C20/C22 40-50% and Alkylpolyphosphate 50-60%
- Lot/batch No.: T71241
- Storage condition of test material: at room temperature , in darkness
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 70-75°C
- Boiling point: >300°C
- Vapour pressure: 6.66 x 10-5 Pa.
- Water solubility (under test conditions): <1mg/L



OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: not considered as ready biodegradable

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Pseudokirchnerella subcapitata (reported as Raphidocelis subcapitata)
- Strain: CCAP 278, 4
- Method of cultivation: culltivated in freswater in darkness


ACCLIMATION
- Acclimation period: 4 days
- Culturing media and conditions (same as test or not):yes
- Any deformed or abnormal cells observed: after the 4 days of acclimatation the cells are observed under microscop (meaured and morphology analysing)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
After each 24h period the concentration of Algae was controled by particle counter

Test conditions

Test temperature:
21-25°C +- 20°C
pH:
between 6.9 and 7.9
Nominal and measured concentrations:
1; 10 ; 100mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: lfask erlenmeyer (250ml)
- Initial cells density: 10000 algues at the start of the study
- No. of organisms per vessel:10000 per erlenmeyer
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):6
-
GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH:no
- Photoperiod:continuous
- Light intensity and quality:4440 lux -8880


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter


- Range finding study
- Test concentrations: 1; 10 ; 100 mg/L
- Results used to determine the conditions for the definitive study:OEDCD guideline 23
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Results with reference substance (positive control):
the latest test (june 2008) gives an result of EC50(72h)=0.69mg/L
Reported statistics and error estimates:
LOD =0.015mg/L
LOQ=0.05mg/L

Any other information on results incl. tables

Toxicity of the test item LCE07105 was performed on the freshwater unicellular green algae "Pseudokirchneriella subcapitata" according to the OECDE 201 guideline (screening test). Results obtained show a slight toxicity of the test item with an inhibition of the average specific growth rate in comparison to the control 50% at the highest concentration of 100mg/L. For the reason, the EC50(0-72h) is superior to the highest tested concentration. In order to control and confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study.

 Nominal test item concentration in mg/L

    total [P]

 

 at 0h

 at 72h

 filtered control

 

 

100mg/L 

 detected 0.056mg/L(indicative value: 0;046mg/L)

 detected 0.056mg/L(indicative value: 0;046mg/L) 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50(72h)>100mg/L in a limit test condition
Executive summary:

Toxicity of the test item LCE07105 was performed on the freshwater unicellular green algae "Pseudokirchneriella subcapitata" according to the OECDE 201 guideline (screening test). Results obtained show a slight toxicity of the test item with an inhibition of the average specific growth rate in comparison to the control <50% at the highest concentration of 100mg/L. For the reason, the EC50(0-72h) is superior to the highest tested concentration. In order to control and confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study.

 Nominal test item concentration in mg/L     total [P]
   at 0h  at 72h

 filtered control

 <LOD  <LOD
100mg/L   detected <0.056mg/L(indicative value: 0;046mg/L)  detected 0.056mg/L(indicative value: 0;046mg/L)