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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October - December 1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Data is from experimental study report

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
An ocular irritation study was conducted on New Zealand White rabbit to assess its eye irritation potential.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
6-amino-4-hydroxynaphthalene-2-sulphonic acid
EC Number:
202-000-8
EC Name:
6-amino-4-hydroxynaphthalene-2-sulphonic acid
Cas Number:
90-51-7
Molecular formula:
C10H9NO4S
IUPAC Name:
6-amino-4-hydroxynaphthalene-2-sulphonic acid
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): 6-amino-4-hydroxynaphthalene-2-sulphonic acid
- Molecular formula : C10H9NO4S
- Molecular weight : 239.26g/mol
- Substance type: Organic
- Physical state: solid
- Purity: No data
- Impurities (identity and concentrations):No data

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
not specified

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
0.1 g (100mg)of sample
Duration of treatment / exposure:
not specified
Observation period (in vivo):
24 and 72 h
Number of animals or in vitro replicates:
1 rabbit

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
animal #1
Time point:
24 h
Score:
ca. 1
Reversibility:
fully reversible within: max. 48 h
Remarks on result:
other: very weak hyperemia
Irritation parameter:
overall irritation score
Basis:
animal #1
Time point:
72 h
Score:
0
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No eye irritation was observed in treated rabbit.

Applicant's summary and conclusion

Interpretation of results:
other: non-irritating
Conclusions:
Slight hyperemia was observed in treated rabbit but the effects were not persisted up to 72 hours, hence the test chemical was considered to be not irritating to the eyes of treated New Zealand White rabbit.
Executive summary:

An ocular irritation study was conducted on New Zealand White rabbit to assess its eye irritation potential.

Approx 100mg of undiluted test chemical was instilled into the eye of rabbit and observed for damage of cornea and mucosa (hyperemia, edema) at 24, 48 and 72 hours.

Slight hyperemia was observed in treated rabbit but the effects were not persisted up to 72 hours, hence the test chemical was considered to be not irritating to the eyes of treated New Zealand White rabbit.