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Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from J-CHECK and EnviChem authoritative database
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Principles of method if other than guideline:
Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I).
GLP compliance:
not specified
Oxygen conditions:
not specified
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 mg/l
Duration of test (contact time):
14 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Parameter followed for biodegradation estimation:
other: BOD and UV-Vis
Details on study design:
- Test equipment: standard
Key result
Parameter:
% degradation (O2 consumption)
Remarks:
and BOD
Value:
5.3
Sampling time:
14 d
Remarks on result:
other: Other details not known
Key result
Parameter:
% degradation (TOC removal)
Value:
0.4
Sampling time:
14 d
Remarks on result:
other: Other details not known
Key result
Parameter:
% degradation (test mat. analysis)
Remarks:
and UV-Vis
Value:
< 0
Sampling time:
14 d
Remarks on result:
other: Biodegradation was negative value
Details on results:
Test substance undergoes 5.3, 0.4 and 0% degradation by BOD, O2 consumption, TOC removal, Test material analysis and UV-Vis parameter in 14 days.
Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The percentage degradation of test chemical was determined to be 5.3, 0.4 and < 0% by BOD, O2 consumption, TOC removal, Test material analysis and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in water.
Executive summary:

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 5.3, 0.4 and < 0% by BOD, O2 consumption, TOC removal, Test material analysis and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical (authoritative databases, 2018). The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 5.3, 0.4 and < 0% by BOD, O2 consumption, TOC removal, Test material analysis and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Various experimental key and supporting studies of the test chemical were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study from authoritative databases (2018),biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 5.3, 0.4 and < 0% by BOD, O2 consumption, TOC removal, Test material analysis and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

Another biodegradation study (from peer reviewed journal GREIM H. et. al., 1994 and handbook, 2008) was conducted for 28 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test). Initial test chemical conc. used for the study was 100 mg/l. The percentage degradation of test chemical was determined to be 7, 6 and 9% by DOC removal parameter after 7, 14 and 28 days, respectively. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

In a supporting study, biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test chemical (from peer reviewed journal GREIM H. et. al., 1994 and handbook, 2008). The study was performed according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test). Municipal sludge was used as a test inoculum for the study. Initial test chemical conc. used for the study was 80 mg/l. The percentage degradation of test chemical was determined to be 0% by ThOD parameter after 20 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in water nature.

 

On the basis of above results for test chemical, it can be concluded that the test chemical can be considered to be not readily biodegradable in nature.