Diaminotoluene

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

short-term repeated dose toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Does not meet important criteria of today standard methods.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The vapour of the test substance was blown into the experimental enclosure of 0.4 m3.  The rats weighed 172-182 g, and 20 were used. The rats were observed daily, weighed weekly, and clinically examined at the end of the exposure.  Blood samples from 5 rats were examined, and liver and kidney function tests were conducted on groups of 5 rats. At the end of the exposure the rats were anaesthetized with ether, and bled to death.  The dissected animals were macroscopically examined.  Various organs of the killed rats were weighed.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diaminotoluene

EC Number:

246-910-3

EC Name:

Diaminotoluene

Cas Number:

25376-45-8

Molecular formula:

C7H10N2

IUPAC Name:

3-methylbenzene-1,2-diamine

Details on test material:

2,4/2,6-Toluylendiamine (80/20)

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Analytical verification of doses or concentrations:

yes

Duration of treatment / exposure:

28 days

Frequency of treatment:

4 hr/day, 5 consecutive days/week

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 0.083 mg/l.
Basis:
analytical conc.

No. of animals per sex per dose:

20 for dose group; animal number of control group not given

Control animals:

yes, sham-exposed

Results and discussion

Effect levels

Dose descriptor:

LOAEC

Effect level:

0.083 mg/L air (analytical)

Sex:

male

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

At a concentration of 0.083 mg/l (83 mg/m3) the exposed rats were similar to the

controls in appearance and condition. The exposed rats had reduced body weight

gain. The blood tests showed that the exposed rats had relative lymphopenia. The

liver and kidney function tests were normal. Dissection of the killed rats did 

not find any compound related changes. The liver, kidney, and thyroid/body weight

ratios were increased.

In another similar study using a concentration of 0.0095 mg 2,4/2,6-TDA/l (9.5 mg/m3) the treated rats did not significantly differ from the control animals.