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EC number: 915-730-3
CAS number: -
Iso E Super was tested for its effect on the activity of soil microflora
(nitrogen transformation) according to OECD TG 216 Nitrogen
Transformation Test. Nitrogen-transformation in soil enriched with
lucerne meal was determined. Test item treated soil was compared with a
non-treated soil. NO3 -nitrogen formed in the nitrification process was
determined by continuous flow analysis. The following test rates of Iso
E Super were used: 3.16, 10, 31.6, 100 and 316 mg test item/kg soil dw.
Sodium chloride was used as reference item. Moisture content was 47% to
48% of WHCmax and temperature was 20°C ± 2°C in the dark. No adverse
(deviations to control below 25%) of the test item on nitrate content in
soil were observed at day 28 up to a concentration of 100 mg test
item/kg soil dw. The soil nitrate formation rates per day did not exceed
the trigger range of ± 25% from the 0 - 28 day interval up to a
concentration of 100 mg test item/kg soil dw. In conclusion, Iso E Super
had no impact on nitrogen transformation (nitrate content and nitrate
formation rate) of soil microorganisms when applied up to 100 mg test
item/kg soil dw treatment. An application of 316 mg test item/kg soil dw
caused a significant increase above the trigger value for nitrate
content and nitrate rate. The NOEC appears to be 100 mg test item/kg
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