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EC number: 915-730-3
CAS number: -
For three different sediment species representing different living and
feeding conditions, the NOEC in a 28d-test is available. The lowest
NOEC, based on measured concentrations, is 17.1 mg/kg dw (Total Organic
Three invertebrate species associated with
the sediment and representing different living and feeding conditions,
were tested according to or in line with OECD TG 218 in compliance with
GLP. The results of these tests are summarised in the below table. More
information on the tests is provided below the table.
Results (mg/kg dw) and 95% confidence limits
Comparable to OECD 218
28d-NOECreprod.& 28d-NOECbiomass= 33.3 mg/kg
28d-EC50reprod. = 97 mg/kg.
Egeler and Gilberg 2005c
Total organic carbon 2.29%
nom. 33.3 mg/kg
~measured 17.1 mg/kg
28d-NOECsurvival& 28d-NOECbiomass= 40.6 mg/kg
28d-EC50survival= 198 <115 – 339> mg/kg
Egeler and Gilberg 2005b
Total organic carbon 2.33%
nom. 40.6 mg/kg,
~measured 18.4 mg/kg
28d-NOECemergence ratio= 400 mg/kg
28d-NOECratite midges= 200 mg/kg
28d-EC50emergence ratio= 642 <516-797> mg/kg
Egeler and Gilberg 2005a
Total organic carbon 2.24%
nom. 200 mg/kg,
~measured 102 mg/kg
The resulting NOECs are expressed based on the measured
concentrations as indicated in the third column of the above table. For
the safety assessment, the results are to be converted to a sediment
containing 5% TOC in order to be comparable to the estimations for a
The sediment was formulated from quartz sand, kaolinite clay and
Sphagnum moss peat. Urtica powder was added as feed. The total organic
carbon content was 2%. The sediment including the feed was spiked with
test material solved in acetone. Each vessel contained a layer of
sediment and the water volume was circa 4 times the sediment volume. The
test animals were introduced after an equilibrium period of 1 week. Test
concentrations were measured in the sediment, porewater as well as in
In the test with Lumbriculus variegatus, the test organisms were
exposed to nominal concentrations of 1.2, 3.7, 11.1, 33.3 and 100 mg/kg
sediment dry weight. The actual concentrations in the sediment upon
introduction of the test organisms were 52% of nominal. On day 14 the
measured concentrations were within 80% of the concentrations on day 0.
This was also the case for the highest concentration on day 28, but
middle concentration fell below 80% (Belfroid and Blok 2006). Survival
was not affected up to the highest test concentration of 100 mg/kg dw.
Reproduction and growth were not affected up to 33 mg/kg dw. The
reproduction was inhibited by 55% and growth was inhibited by 35% at 100
mg/kg (Egeler and Gilberg 2005). After correction for the actual
concentration on day 0, the actual NOEC was 17.1 mg/kg dw (Belfroid and
In the test with Chironomus riparius, the test organisms were
exposed to nominal concentrations of 50, 100, 200, 400 and 1000 mg/kg dw
of test material in the sediment. The actual concentrations in the
sediment on day 0 were 50 to 67% of the target concentrations. At
termination of the test the concentration was 84-89% of the
concentration on day 0 (Belfroid and Blok 2006). The emergence ratio was
not affected up to 400 mg/kg, whereas the development rate was not
affected in any of the test concentrations. At concentrations ≥ 400
mg/kg(d.w.) an increasing percentage of fully emerged midges were dead
and/or not able to fly (ratite) and also the percentage of dead,
incompletely emerged midges increased at 400 - 1000 mg/kg (Egeler and
Gilberg 2005a).Therefore the NOEC was 200 mg/kg dw (nominal); after
correction for the concentrations on day 0, the actual NOEC was 102
mg/kg dw (Belfroid and Blok 2006).
In the test with Hyalella azteca, the test organisms were exposed
to nominal concentrations of 1.2, 4.0, 12.7, 40.6 and 130 mg/kg sediment
dry weight. The actual concentrations on day 0 of the test with Hyalella
azteca were 43 to 47% of the intended concentrations in the sediment. On
day 28 the concentrations were within 80% of the concentrations on day 0
(Belfroid and Blok 2005). Survival and biomass were not affected up to
40.6 mg/kg dw (NOEC), whereas growth was not affected up to the highest
concentration of 130 mg/kg dw (Egeler and Gilberg 2005b). After
correction for the concentrations on day 0 the actual NOEC was 18.4
mg/kg dw (Belfroid and Blok 2006).
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