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EC number: 915-730-3
CAS number: -
The earthworm Eisenia foetida was tested.The
test material at appropriate concentrations were dissolved in equal
amounts of acetone, mixed with the quartz sand and allowed to slowly
evaporate and mixed with the standard soil containing
10% Sphagnum peat, 20% kaolinite clay, approximately 70% fine
quartz-sand (grain size 0.05 -0.2 mm) and 0.4% calcium carbonate to
adjust to pH 6.0±0.5.The earthworm test was carried
out according to OECD 222 and ISO 11268. Adult worms (Eisenia fetida)
were exposed to nominal concentrations in soil of 3.16, 10, 31.6, 100
and 316 mg/kg. Weights of the adult worms ranged between 306 and 594 mg.
The worms were fed weekly with finely ground cattle manure. Adult worms
were removed after 4 weeks of exposure, counted and weighed. The
remaining offspring remained in the test containers for another four
weeks. Mortality of the adults was not affected after 4 weeks in
concentrations up to 100 mg/kg. Body weight was significantly reduced in
the 100 mg/kg group. Reproduction was significantly affected at a
concentration of 100 mg/kg (30.6% inhibition, LOEC). In the highest
concentration of 316 mg/kg reproduction was not assessed because of the
low survival rate of adults.
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