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EC number: 946-101-1
CAS number: -
72h-EC50 for cell growth inhibition and growth rate reduction based on
TWA concentrations were both determined to be >0.017 mg/L i.e. exceeded
the maximum soluble concentration of test substance in test medium at a
loading rate of 100 mg/L. The NOEC for both the parameters was
considered at 0.017 mg/L (TWA concentrations).
A study was conducted to determine the
toxicity of the test substance to algae and cyanobacteria according to
OECD Guideline 201 and EU Method C.3, in compliance with GLP. A combined
limit/range-finding test (test type: static) was performed, during
which, six replicates of exponentially growing algal cultures
(Pseudokirchneriella subcapitata) were exposed to a control and to a WAF
prepared at a loading rate of 100 mg/L. In addition, three replicates
per group were exposed to WAFs prepared at 1.0 and 10 mg/L. The initial
algal cell density was 10E04 cells/mL and the total exposure period was
72 h. The test concentrations were analytically verified and the pH,
temperature, cell density and appearance were recorded throughout the
study. At the start of the test, the actual test concentrations were
0.044 and 0.101 mg/L in the WAF prepared at 100 mg/L and incubated with
and without algae, respectively. At the end of the exposure period, the
measured concentrations had dropped to 21-23% of the initial
concentrations. Based on these results, the Time Weighted Average (TWA)
concentrations were calculated and used to determine the effect
parameters. The TWA concentrations were estimated to be 0.017 and 0.039
mg/L in samples with and without algae, respectively. No significant
differences were recorded between the values for growth rate or yield at
any of the test concentrations when compared to the control group.
Microscopic observations at the end of the test revealed a normal and
healthy appearance of the algal cells exposed to the highest test
concentration when compared to the control. The study met the
acceptability criteria prescribed by the study plan and was considered
valid. Under the study conditions, no significant differences were
recorded between the values for growth rate or yield for
Pseudokirchneriella subcapitata at any of the test concentrations. Both
the EC50 for growth rate inhibition (72h-ERC50) and the yield inhibition
(72hEYC50) were beyond the range tested (EC50: >0.017 mg/L), i.e.
exceeded the maximum soluble concentration of test substance in test
medium at a loading rate of 100 mg/L. The 72h-NOEC for both growth rate
inhibition and yield inhibition was 0.017 mg/L (TWA concentration
corresponding to a loading rate of 100 mg/L) (Augusiak, 2017).
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