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EC number: 946-101-1
CAS number: -
The test substance was considered not
study was conducted to determine the ready biodegradability of the test
substance according to OECD Guideline 301B and EU Method C.4 -C (CO2
evolution test), in compliance with GLP. Domestic activated sludge was
exposed to the test substance at concentrations of 0 or 17.5 mg/L (i.e.,
corresponding to 12 mg TOC/L) for 28 days. A positive control (sodium
acetate) and a toxicity control (test substance + sodium acetate) were
included in the experiment followed by sampling at regular intervals to
measure the CO2 evolution. The amount of CO2 produced was determined by
titrating the remaining Ba(OH)2 with with standardised HCl. Each sample
was measured in duplicates. The relative biodegradation values
calculated from the measurements performed during the test period
revealed 14% and 15% biodegradation of the test substance (based on
ThCO2), for the duplicate bottles tested. Thus, the criterion for ready
biodegradability (at least 60% biodegradation within a 10-day window)
was not met. In the toxicity control, the test substance was found not
to inhibit microbial activity. Since all criteria for acceptability of
the test were met, this study was considered to be valid. Under the
study conditions, the test substance was considered not readily
biodegradable (Desmares-Koopmans, 2017).
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