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EC number: 250-797-6
CAS number: 31775-16-3
In a publication regarding the development of a screening test for
inhibitory effects of dye-stuffs on aerobic waste-water bacteria, later
used as the methodical basis of the OECD 209 activated sludge
respriation inhibition test, the toxicity of the submission substance to
aerobic waste water bacteria had been tested. The EC50 (3 hours) had
been determined as > 100 mg/L (defined as at least 80% respiration rate
of the control) . This is probably a nominal concentration, however no
specific indication is given. The sludge used by the different
laboratories was predominantly domestic (4 laboratories used domestic,
one of five laboratories however used industrial sludge). It is not
clear, which type of sludge had been used with the submission substance.
No information on substance purity is given.
However, as this interlaboratory study formed the basis for OECD
guideline 209, the data are judged to be reliable (reliability category
2). From these data, the submission substance would be regarded as not
harmful to aerobic microorganisms of sewage treatment plants.
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