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EC number: 250-797-6
CAS number: 31775-16-3
growth (area under growth curve) and growth rate exposure
Growth inhib. [%]
Growth rate reduct. [%]
purpose of this study was to determine the effects of the test substance on
the growth of a unicellular green algae species.
growing cultures of Desmodesmus
exposed to 100 mg/L of the test
substance and a control (0 mg/L) over several generations under defined
cell density in each solution was measured at least every 24 hours. The
inhibition of growth in relation to a control culture was determined.
to Directive 92/69/EEC, Annex Part C, C.3. Algal Inhibition Test, it is
sufficient to conduct a limit test with a test substance concentration
of 100 mg/L if the inhibition of the biomass
and growth rate is < 25 % compared to the control.
test substance was weighed into a 250 mL beaker, diluted with algal
medium, transferred to a
500 mL Erlenmeyer flask and filled up to final volume of 500 mL. After
treatment with ultrasonic
waves for approx. 30 minutes the preparation was stirred for
approximately 4 days to ensure
that the solubility limit of the test substance in the test water was
reached. The resulting dispersion
was filtered through a membrane filter of 0.45 µm pore size. The
resulting solution was
visually clear. No particulate matter was observed.
requested by the sponsor analytical monitoring of the test substance
concentrations in the test
medium was not conducted, since no relevant results could be expected.
were observed microscopically at the beginning and at the end of the
study. No visible changes
of the shape of the algae were noticed in either the control or the
the 100 mg/L treatment the inhibition of the biomass was 2 % and the
growth rate reductionwas
3 % compared to the control.
resulted in the following effect concentrations:
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