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EC number: 250-797-6
CAS number: 31775-16-3
submission substance was
tested in zebrafish (Danio rerio) for 96 hours in
a static system.
The test was performed
with GLP compliance and following relevant guidelines with reliability
concentration tested was 100 mg/L.
nominal concentration of 100 mg/L was used for the preparation of a test
solution at maximum
saturation of the test substance. After treatment with ultrasonic waves
for approx. 30 minutes the
preparation was stirred for approximately 4 days to ensure that the
solubility limit of the test substance in the test water was reached.
The resulting dispersion
was pre-filtered through a cellulose filter followed by filtration
through a membrane filter of 0.45 µm pore size.
concentration was visually present as clear solution. Analytical
monitoring of the concentration of the test substance in the test water
was not conducted since no relevant results could be expected. The
solubility limit of the test
substance was below the analytical detection limit. From the properties
of the test substance it
could be concluded that the test substance is chemically stable in water over
the test interval of 96 hours.
this 96-hour acute toxicity study,
100 mg/L group showed no effect in comparison to the control. In the
no effects were observed.
Under the conditions
of this test the LC50 of the submission substance after
96 hours was > 100 mg/L (based on nominal concentration) and is far
above the solubility limit of the test substance in the test water.
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