Registration Dossier
Registration Dossier
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EC number: 200-522-0 | CAS number: 61-90-5
Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2006
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: The results of this study are presented in an extended abstract.
Data source
Reference
- Reference Type:
- publication
- Title:
- The effects of branched-chain amino acids on canine neoplastic cell proliferation and death
- Author:
- Wakshlag et al.
- Year:
- 2 006
- Bibliographic source:
- Journal of Nutrition 136:2007S-2010S
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- not specified
- Type of method:
- in vitro
- Endpoint addressed:
- other: cell proliferation
Test material
- Reference substance name:
- L-leucine
- EC Number:
- 200-522-0
- EC Name:
- L-leucine
- Cas Number:
- 61-90-5
- Molecular formula:
- C6H13NO2
- IUPAC Name:
- L-leucine
Constituent 1
Administration / exposure
Doses / concentrations
- Remarks:
- Doses / Concentrations:
5, 10, 50, 100 mM
Basis:
nominal conc.
Results and discussion
Applicant's summary and conclusion
- Conclusions:
- Leucine results in a reduction of cell proliferation of dog cells at concentrations higher than in vivo plasma leucine concentrations.
- Executive summary:
Leucine, valine, isoleucine and arginine were tested for their effect on the cell proliferation of canine osteosarcoma,
canine bronchoepithelial carcinoma and
Madin-Darby canine kidney (MDCK) cells. All amino acids tested had anti-proliferative capacities at the highest concentration (100 mM) but leucine and arginine were far more suppressive at lower concentrations at 50 and 10 mM than valine or isoleucine. The concentrations of leucine which result in a reduction in cell proliferation are higher than in vivo leucine plasma concentrations.
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