Registration Dossier

Administrative data

Endpoint:
specific investigations: other studies
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2006
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: The results of this study are presented in an extended abstract.

Data source

Reference
Reference Type:
publication
Title:
The effects of branched-chain amino acids on canine neoplastic cell proliferation and death
Author:
Wakshlag et al.
Year:
2006
Bibliographic source:
Journal of Nutrition 136:2007S-2010S

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified
Type of method:
in vitro
Endpoint addressed:
other: cell proliferation

Test material

Reference
Name:
Unnamed
Type:
Constituent

Administration / exposure

Doses / concentrations
Remarks:
Doses / Concentrations:
5, 10, 50, 100 mM
Basis:
nominal conc.

Results and discussion

Applicant's summary and conclusion

Conclusions:
Leucine results in a reduction of cell proliferation of dog cells at concentrations higher than in vivo plasma leucine concentrations.
Executive summary:

Leucine, valine, isoleucine and arginine were tested for their effect on the cell proliferation of canine osteosarcoma,

canine bronchoepithelial carcinoma and

Madin-Darby canine kidney (MDCK) cells. All amino acids tested had anti-proliferative capacities at the highest concentration (100 mM) but leucine and arginine were far more suppressive at lower concentrations at 50 and 10 mM than valine or isoleucine. The concentrations of leucine which result in a reduction in cell proliferation are higher than in vivo leucine plasma concentrations.