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EC number: 208-796-3
CAS number: 542-02-9
No significant reduction of growth rate or inhibition of yield was recorded at any of the concentrations of Acetoguanamine tested. Both the EC50 for growth rate reduction and the EC50 for yield inhibition exceeded an analytically confirmed nominal concentration of 100 mg/l. The NOEC for growth rate reduction and yield inhibition was >= 100 mg/l.
The 72 -h EC50 value of Acetoguanamine in Desmodesmus
subspicatus determined according to OECD 201 resp. EU C.3.
The study was performed in the test system using five
concentrations ranging from 4.6 to 100 mg/L.The cell concentration of
each replicate was determined by measuring the absorption of the
solutions at 440 nm every 24 hours with a spectral photometer. The cell
density of the cultures was calculated based on the correlation curve
between the adsorption and the cell density of the cultures determined
by microscope counts. Growth rate µ, area under the growth curve (AUC)
and the yield were determined from the cell densities at the respective
observation times.Marginal, but statistically significant inhibition of
algal growth was observed at the highest concentration of 100 mg/L
regarding the endpoint AUC. The other endpoints showed no significant
inhibition. At the start and at the end of the test, the content of the
test item in the test solutions was determined using HPLC. The recovery
after 72 hours was in a range of 102 % up to 105 % of the start
concentration. The measured concentrations lay between 90 % and 100 % of
the nominal concentration at the beginning of the test and between 93 %
and 105 % of the nominal concentration at the end of the test.
Therefore, the determination of the results was based on the nominal
concentrations. The EC50 values of potassium dichromate were tested in a
separate reference test (GLP study no. 201206R301). The values lay
within the normal range of the laboratory.
The following results for the test item Acetoguanamine were
> 100 mg/L
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