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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2002-08-26 to 2002-08-30
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Nominal concentrations: 0 (Control) and 100 mg/L

- Sampling method: Duplicate samples of the test media were taken at the start and end of the test.

- Sample storage conditions before analysis: Samples were analysed immediately after collection.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: A nominal concentration of 100mg/l was prepared by mixing 602 mg of test substance with 6 L of dilution water, and stirring for 71.5 hours

- Controls: Dilution water
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM

- Common name: Rainbow Trout

- Source: Forellenzuchtbetrieb, Tautenhahn, Trostadt, Germany

- Length at study initiation (length definition, mean, range and SD): 4.3 (+/- 0.3) cm

- Weight at study initiation (mean and range, SD): 0.7 (+/- 0.2) g

- Feeding during test: no

ACCLIMATION

- Acclimation period: 14 days

- Acclimation conditions: same as test

- Type and amount of food: Commercial fish diet
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
250 mg/l as CaCO3
Test temperature:
16 - 17 ⁰C
pH:
7.6 - 8.1
Dissolved oxygen:
≥8.4 mg/L
Salinity:
not applicable
Nominal and measured concentrations:
Nominal concentrations: 0 (control) and 100 mg/L

Measured concentrations at the start of the test in the 100 mg/L nominal test medium treatment were 103% of nominal. At the end of the test, the measured concentration was between 74 and 77% of the nominal value. The mean measured concentration during the test was 90% of nominal.

Test results are expressed relative to nominal concentration.
Details on test conditions:
TEST SYSTEM

- Test vessel: aquaria

- Type: open

- Material, size, fill volume: glass, 8 L, 5.5 L test medium

- Aeration: Yes

- Renewal rate of test solution (frequency/flow rate): static test

- No. of organisms per vessel: 7

- No. of vessels per concentration (replicates): 1

- No. of vessels per control (replicates): 1

- Biomass loading rate: 0.9 g/L


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Re-constituted fresh water prepared by adding analytical-grade salts to deionised water.

- Alkalinity: 0.8 mmol/L

- Ca/mg ratio: 4:1

- Culture medium different from test medium: No

- Intervals of water quality measurement: Daily


OTHER TEST CONDITIONS

- Adjustment of pH: no

- Photoperiod:6 h light; 18 h dark

- Light intensity: 560-800 lux


EFFECT PARAMETERS MEASURED: Mortality. After 2, 24, 48, 72 and 96 h.


TEST CONCENTRATIONS

- Spacing factor for test concentrations: Limit test

- Range finding study: yes, not reported
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
but exposure is to hydrolysis products
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
but exposure is to hydrolysis products
Basis for effect:
mortality
Details on results:
- Mortality of control: 0
Reported statistics and error estimates:
No toxic effects were observed in the test and therefore statistical analysis of the results was not required.

Table 1. Results of analysis of test media 

Nominal concentration of test substance (mg/L)

Measured test substance concentration at start of test (mg/L)*

Measured test substance concentration after 96 hours (mg/L)*

Nominal concentration of substance taking percentage of active ingredient into account (mg/L)**

Measured test substance concentration at start of test as percentage of nominal

Measured test substance concentration after 96 hours as percentage of nominal

0 (Control)

Not applicable

Not applicable

Not applicable

Not applicable

Not applicable

100 (Rep 1)

98.82

71.16

95.92

103

74

100 (Rep 2)

99.24

73.48

95.92

103

77

*Calculated from measured Dissolved Organic Carbon (DOC) concentration and taking percentage of carbon in the test substance and molecular weight (237.326) of the substance into account.

**Percentage active ingredient of the substance is 95.92%

There were no toxic effects at the only nominal test concentration of 100 mg/l.

Validity criteria fulfilled:
yes
Conclusions:
A 96-hour LC50 value of >100 mg/L and a NOEC of ≥100 mg/L have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss based on nominal exposure concentrations of the substance. The test substance hydrolyses in water and it is therefore likely that the test organisms were primarily exposed to the hydrolysis products of the substance.

Description of key information

LC50 (96 h) > 100 mg/L (nominal) based on mortality of Oncorhynchus mykiss (OECD 203)

Key value for chemical safety assessment

Additional information

One experimental study is available investigating the toxicity of methyl-N-[3-(trimethoxysilyl)propyl]carbamate (CAS 23432-62-4) to freshwater fish (2002).

A 96-hour LC50 value of >100 mg/Lwas determined for the effects of the test substance on mortality of Oncorhynchus mykiss based on nominal exposure concentrations of the substance. Since the substance is expected to hydrolyse rapidly in contact with water (DT50: 3.0 h at 20 - 25 °C, pH 7), the result from this study mainly investigated the possible effects of the hydrolysis products (Methyl-N-[3-(trihydroxysilyl)propyl]carbamate and methanol).