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EC number: 250-418-4
CAS number: 30989-05-0
NOEC = 1000 mg/L; EC10 and EC50 > 1000 mg/L
the GLP compliant (key) study from BASF (1999) the
effect of B-TEGME on the oxygen consumption of activated sludge was
determined in a limit test with 1000 mg B-TEGME/L. The study is
considered compliant with OECD 209, ISO 8192 and C.11. The inoculum was
obtained from laboratory wastewater plants treating municipal waste
water. The inoculum was washed, adjusted to 5g dry mass/L and
subsequently aerated for 24 hours. Three controls and one replicate for
the treated test solution was set up. Non-replicated positive controls
were set up with 1, 10 and 100 mg 3,5 -dichlorophenol/L The respiration
measurements were performed 30 minutes after start of the exposure.
test with 3,5 -dichlorophenol resulted in an EC50 of 8.8 mg 3,5
-dichlorophenol/L. The variation between 3 controls was < 15%. The
findings from the positive controls render the test as valid. At 1000 mg
B-TEGME/L, the respiration was increased by 6% relative to the average
respiration in the control. Therefore,
the EC0 = 1000 mg B-TEGME/L and the EC10, EC20, EC50 and EC80 are > 1000
In the GLP compliant (supporting) study from
Shell (1986), the microbial inhibition of a brake fluid (which contains
17% B-TEGME) to Pseudomonas fluorescens was determined in a microbial
inhibition test. The test design was based on the method described in
"Degradability, Ecotoxicity, and Bioaccumulation, TNO, Delft, The
Netherlands, 1977". The
nominal test concentrations were 0 (Control), 10, 32, 100, 320,
and 1000 mg brake fluid/L (nominal concentrations), corresponding to 0
(control), 1.7, 5.44, 17, 54.4, and 170 mg B-TEGME/L, respectively.
Sodium pentachlorophenate was used as positive control. Growth curves
were constructed of the optical density of the inoculated media versus
time and the rate determined as the slope of the exponential growth
phase. The reference substance, sodium pentachlorophenate inhibited with
an EC50 =18 mg/L while the brake fluid caused a maximum of 19%
inhibition at 1000 mg brake fluid/L, corresponding to 170 mg B-TEGME/L,
the highest concentration tested. Therefore, the EC50 is > 170 mg
B-TEGME/L. The exposure duration, environmental conditions like
temperature and details on the measurements of the optical density and
the origin of the cultures were not reported. Due
to the limited information provided the results of this study are
considered to have only limited relevance and the study was considered
to be supporting.
GLP (key) study with B-TEGME (BASF, 1999) provided an EC20 and EC50 of >
1000 mg B-TEGME/L. The advantage of this study is that it was performed
with B-TEGME itself and not with a B-TEGME containing brake fluid as in
the study from Shell (1986) where
other additives may have affected microbial growth. The
(supporting) study from Shell (1986) provided
an EC50 of > 170 mg B-TEGME/L, the highest tested concentration. Due to
the above restrictions the test results from Shell (1986) were
not considered as relevant for the risk assessment.
conclusion, the EC10 and EC50 values of >1000 mg B-TEGME /L are
considered reliable and sufficiently conservative to assess the toxicity
of B-TEGME on microorganisms.
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