Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 250-418-4
CAS number: 30989-05-0
LC50 > 222.2 mg/L Oncorhynchus mykiss acute (OECD 203)
the assessment of the acute toxicity on freshwater fish one key study (Shell
1999a) and one supporting study (Shell1987 a) are available, both with
B-TEGME containing mixtures.
the GLP compliant key study of Shell (1999a), the acute toxicity of
methyl borate esters (containing 22% B-TEGME) was investigated on O.
mykiss. The EU C.1 and OECD 203 guideline compliant limit test was
performed as 96 hour semi-static study with 24 hour renewal intervals. No
relevant restrictions appeared and the only disadvantage of this study
is the fact that the study was performed with a B-TEGME containing
mixture and not with B-TEGME as pure substance itself. The
effective test concentration was 222.2 mg B-TEGME/L (applied as 1010 mg
methyl borate esters/L, mean measured concentration). During the 96
hours of exposure, no adverse effects were observed in the control and
the treated test group. Hence, the 24, 48, 72, and 96-hour LC50 were >
222.2 mg B-TEGME/L.
the GLP compliant supporting study of Shell (1987a), the acute toxicity
of a brake fluid (containing 17% B-TEGME) was investigated on O.
mykiss. The study was performed as 96 hour semi-static test with 24
hour renewal intervals. The study was not performed according to a
specific guideline, but the test design was similar to OECD 203.
Following restrictions apply: (1) dose verification analysis was not
performed, and (2) the temperature variation of 3.5°C (13.5 to 17.0°C)
exceeded the maximum variation of 2°C allowed by modern EU, OECD and
OPPTS test guidelines. Nominal test concentrations were: 213, 533 and
1065 mg brake fluid/L, equivalent to 36.2, 90.6 and 181.1 mg B-TEGME/L.
After 96 hours of exposure, 0, 0, 2 and 10 out of 10 fish had died in
the control, 36.2, 90.6 and 181.1 mg B-TEGME/L treatment groups,
respectively. The 96-hour LC50 was 100.3 mg B-TEGME/L.
results of the key study show that the LC50 fish was > 222.2 mg
B-TEGME/L. The results of the supporting study show that the LC50 of
B-TEMGE was 100.3 mg B-TEGME/L.
following reasons, the LC50 of > 222.2 mg B-TEGME/L is considered
reliable and sufficiently conservative to assess the toxicity of B-TEGME
on fish: (1) the
above mentioned restrictions of the supporting study (no analytical
dose-verification, temperature deviation) may lead to less reliable
results and (2) other
additives of the brake fluid may have caused the mortality in the
above LC50 value is higher than the limit for classification and
labeling, i. e., higher than 100 mg B-TEGME/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again