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EC number: 213-254-4
CAS number: 932-64-9
There is no study available however the registrant has reported cases of workers with throat irritations when breathing NTO dust.
There was no change in the test substance, NTO/MTT solution or the water
control/MTT solution after three hours incubation in the dark at 37 +/-
2oC in a humidified atmosphere of 5% CO2 in air. The test substance had
not interacted with the MTT.
Negative control: the mean absorbance of the triplicate negative control
values was 0.916 which was between the minimum and maximum values of 0.6
and 1.5. The standard deviation (SD) of the % viability was 7.6 which
was below the maximum value of 18.
Positive control: The percentage mean viability of the positive control
was 14.6 ± 4.2 of the negative control. These were below the maximum
acceptance values of 40% viability and SD of 18.
EPISKIN™ results: The percentage value for the OD of test substance NTO,
linked to colour was 2.074% of the negative control with MTT (Table 1).
As this value was below 5%, correction of the test substance OD values
was not required.
Responses in Rabbits Treated with Test substancea
Time to Score
Wash at 30 sec
Wash at 5 min
rabbits per wash conditions.
cornea response = 80; maximum iris response = 10 ; maximum conjunctivae
response = 20.
*Unable to score cornea
and/or iris for swelling
Justification for selection of skin irritation / corrosion endpoint:
One reliable GLP in vitro study following international guidelines
Justification for selection of eye irritation endpoint:
One in vivo study available
NTO did not cause any irritation in an in vitro EpinSkin study or an
acute dermal study. However, there are reports from the registrant of
workers with skin irritation due to NTO exposure on wet unprotected skin
and throat irritation due to breathing in NTO dust. Therefore NTO is
classified as a skin and eye irritant category 2 and acute inhalation
Category 3 in accordance with Regulation (EC) No 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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