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EC number: 213-254-4
CAS number: 932-64-9
NTO is demonstrated to be ultimately biodegradable by different
microbial species, belonging to various bacteria and fungi. The
substance can totally transformed to urea and a polar compound assumed
to be urea/ hydroxyurea.
The first step of the microbial metabolisation is the
nitroreduction of NTO within 24 hours to ATO. This is followed by a two
week lasting period where the triazolone ring of ATO is cleaved to urea
and probably hydroxyurea as second step. This reaction consists on the
hydrolysis of the pseudo `guanido' group of ATO.
The metabolising strains are adapted to the substance and are
collected from waste water contaminated with NTO. From this source
source two strains could be isolated:
- Penicillium chrysogenum CNCM. I-2081 and
- Bacillus licheniformis CNCM. I-1915
Both strains were able to biodegrade the substance NTO to ATO and
further to urea and hydroxyurea.
Besides, a screening of microorganisms from various microbial
collections including eight bacteria and 22 fungi, three strains were
also able to reduce NTO to ATO. Identified strains are:
- Beauveria bassiana ATCC 7159,
- Rhizopus arrhizus ATCC 11145,
- Cylindrocarpon radicicola ATCC 11011
However, none of these strains have been demonstrated to further
In another paper it was also demonstrated, the substance DNAN can
be degraded by infidigenous microbial communities. DNAN and TNO share
the same nitro-group structure what has been shown before to be
degraded, followed by the cleavage of the also comparable nitrozole
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