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EC number: 247-148-4
CAS number: 25637-99-4
No control group exceeded 10% mortality during the initial 28 days of
Each control replicate produced at least 30 juveniles.
The percent coefficient of variance (CV for the control reproduction was
The effect of Hexabromocyclododecane
(HBCD) on the mortality and reproductive rates of Eisenia
over a 28 day exposure period was examined.
study was conducted using a range of concentrations of HBCD (78.5,
157, 313, 625, 1,250, 2,500, and 5,000 mg HBCD per kg dry soil)
in artifical soil. The adult earthworms were given a 28 day exposure
period in the HBCD test soil and then removed, washed and assessed for
mortality rates. The adult earthworms were then allowed to expunge their
gut contents for 48 hours and then frozen for analysis.
test soil was incubated for a further 28 days to allow un-hatched
offspring to mature. After the 28 day incubation period the number of
juvenile offspring was assessed and the mean reproductive rates of the
Under the conditions of this
study, the 28-day mortality NOEC value was 4,190 mg HBCD per kilogram of
dry soil. The estimated EC10 and EC50 values for
adult earthworm survival were >4,190 mg/kg dry soil. After 28 days of
exposure, measured concentrations of HBCD in the worm tissue samples
were <0.200 (control), 3.40, 7.32, 16.8, 15.3, 510, 71.2, and 150µg HBCD
per gram of tissue. The bioaccumulation factors for the treatment worms
ranged from 2.6 x 10-2 to 6.9 x 10-2. Therefore,
HBCD did not bioaccumulate (i.e., BAF values < 1.0) within the worm
tissues during the 28-day exposure. The 56-day reproduction NOEC value
was 128 mg HBCD per kilogram of dry soil. The estimated EC10 and
EC50 values for average reproduction were 21.6 (95%
confidence limits of 0.000468 to 110 mg/kg) and 771 mg/kg dry soil (95%
confidence limits of 225 to 4,900 mg/kg), respectively.
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