Registration Dossier

Toxicological information

Basic toxicokinetics

Currently viewing:

Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented publication/study report which meets basic scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Metabolism of styrene oxide to styrene glycol by mouse liver and lung
Author:
Carlson G P
Year:
1998
Bibliographic source:
Journal of Toxicology and Environmental Health, Part A, 53:19–27, 1998
Report Date:
1997

Materials and methods

Objective of study:
metabolism
Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Test animals

Species:
mouse
Strain:
other: CD-1 and NSA
Sex:
male/female

Administration / exposure

Route of administration:
other: incubation with microsomes
Vehicle:
other: Tris buffer
Details on exposure:
Microsomes were prepared by ultracentrifugation and were incubated for 20 min in an incubation mixture containing 1 mM styrene oxide in 0.5 M Tris buffer with a total volume of 0.42 ml. Incubations were carried out at 37°C.
Control animals:
no
Positive control:
no
Details on study design:
comparison of two mouse strains with respect to stereoisomers formed

Results and discussion

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
R-enantiomer and S-enantiomer of styrene glycol

Any other information on results incl. tables

Liver

Lung

Strain

R

S

R

S

Racemic

NSA

CD-1

24.97 ± 1.97

26.66 ± 2.08

6.02 ± 0.93

10.34 ± 1.83b

6.33 ± 0.56°

7.44 ± 0.71a

0.96 ± 0.08a

1.32 ± 0.20a

R-SO

NSA

CD-1

26.56 ± 3.23

36.32 ± 1.41b

1.56 ± 0.31

2.22 ± 0.22

5.28 ± 1.00a

3.62 ± 0.68a

1.83 ± 0.89

0.36 ± 0.11

S-SO

NSA

CD-1

0.84 ± 0.28

1.04 ± 0.05

51.16 ± 5.10

26.72 ± 7.97b

0.61 ± 0.10

0.56 ± 0.10

10.49 ± 1.44a

9.09 ± 1.36a

Note.Values are mean ± SE for five animals per group and are nmol styrene glycol formed/mg protein/min.

aSignificantly different (p< .05) from liver for same strain and enantiomer

bSignificant difference (p< .05) between strains.

Applicant's summary and conclusion