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EC number: 607-759-2
CAS number: 25618-55-7
All treated animals survived the scheduled study period.
No clinical signs were observed in any animals of the control group or
group 2 (25%). About 3 hours after the first topical application, a
slight ear erythema was observed at both dosing sites in all mice of
group 4 (100%, undiluted), persisting for a total of four days. On the
second application day, a slight ear erythema was observed at both
dosing sites in all mice of group 3 (50%), persisting for a total of two
In this study Stimulation Indices (S.I.) of 1.4, 2.1, and 1.9 were
determined with the test item at concentrations of 25, 50, and 100 % in
ethanol/water (7:3, v:v), respectively.
In this study the test item dissolved in ethanol/water (7:3, v:v) was
assessed for its possible contact allergenic potential.
For this purpose a local lymph node assay was performed using test item
concentrations of 25, 50 and 100 % by topical application to the dorsum
of each ear lobe (left and right) for three consecutive days. A control
group of four mice was treated with the vehicle only. Five days after
first topical application the mice were injected intravenously into a
tail vein with radio-labelled thymidine. Approximately five hours after
intravenous injection, the mice were sacrificed, the draining auricular
lymph nodes excised and pooled per group. Single cell suspensions of
lymph node cells were prepared from pooled lymph nodes which were
subsequently washed and incubated with trichloroacetic acid
overnight.The proliferative capacity of pooled lymph node cells was
determined by the incorporation of radio-labelled thymidine measured in
a scintillation counter.
The test item was not a skin sensitiser in this assay.
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