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EC number: 413-110-2
CAS number: 135861-56-2
The EC50 value was determined to be > 0.10 mg/L and the No Observed Effect Concentration was ≥ 0.10 mg/L. The EC50 values based on the time-weighted mean measured test concentration were > 0.080 mg/L, and correspondingly the No Observed Effect Concentration was ≥ 0.080 mg/L.
A study was performed to assess the effect of the test material on the
growth of the green alga Scenedesmus subspicatus. The method
followed that described in the OECD Guidelines for Testing of Chemicals
No. 201, "Alga, Growth Inhibition Test" referenced as Method C.3 of
Commission Directive 92/69/EEC.
a preliminary range-finding study, Scenedesmus subspicatus was
exposed to an aqueous dispersion of the test material at a concentration
of 0.10 mg/l (six replicate flasks) for 72 hours, under constant
illumination and shaking at a temperature of 24 ± 1°C.
Samples of the algal populations were removed daily and cell
concentrations determined for each control and treatment group.
Exposure of Scenedesmus subspicatus to the test material gave EC50
values of greater than 0.1 mg/l and correspondingly the No
Observed Effect Concentration was greater than or equal to 0.10 mg/l.
The test concentration of 0.10 mg/L was the highest attainable test
concentration that could be prepared due to the limited solubility of
the test material in water and auxiliary solvent and having due regard
to the amount of auxiliary solvent permitted in the test under the OECD
Analysis of the test solutions at 0 and 72 h showed a marked decline in
test concentrations and so it was considered justifiable to base the
results on the time-weighted mean measured test concentration also. The
EC50, values, based on the time-weighted mean measured test
concentration were > 0.080 mg/l and correspondingly the No Observed
Effect Concentration was ≥ 0.080 mg/l.
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