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EC number: 601-329-8
CAS number: 114798-26-4
pH before addition of sludge
3 h contact time
(mg O2/l h)
(mg O2/g h)¹
% Inhibition respiration relative to the control (mean value)
substance, MK-0954 Free Acid
control of test substance.
amount of suspended solids in the final test mixture was 1.5 g/l.
The influence of MK-0954 Free Acid on the
respiration rate of activated sludge was investigated after a contact
time of 3 hours.
The study procedures described in this report
were based onthe OECD guideline No.
addition, the procedures were designed to meet the test methods of theCouncilRegulation
(EC) No. 440/2008 of, Publication No.
L142, Part C11 and ISO Standard 8192 (2007).
The batch of MK-0954 Free Acid tested was a
white powderand tested as received.The test substance was
not completely soluble in test medium at the loading rates initially
prepared.Therefore, the test substance
– Milli-RO water mixtures were magnetically stirred for a period of
approximately 24 hours. Subsequently, synthetic medium, sludge and
Milli-RO water were added resulting in the required loading rates.
Optimal contact between the test substance and test medium was ensured
applying continuous aeration and stirring during the 3-hour exposure
period. Thereafter, oxygen consumption was recorded for 10-15 minutes.
In a combined limit/range-finding test loading rates of 10, 100
and 1000 mg/l were tested. The highest loading rate was tested in
triplicate, lower concentrations consisted of one replicate. In
addition, blank-controls and a nitrification control of the sludge were
included. Furthermore, an abiotic control and the highest concentration
with a nitrification inhibitor were tested.
limit/range-finding test no
statistically significant inhibition of the respiration rate of the
sludge was recordedat a loading rate of 1000 mg MK-0954
Free Acid per litre(Two
Sample t-Test : α=0.05 Toxstat).
There was no oxygen uptake from abiotic processes and the results
at 1000 mg/l with a nitrification inhibitor showed that the
heterotrophic inhibition of the respiration rate was comparable to the
The batch of activated sludge was checked for
sensitivity by testing the reference substance
3,5-dichlorophenol, which showed normal
The study met the
acceptability criteria prescribed by the protocol and was considered
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