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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

OECD Guideline 301F, EU Method C.4-D, GLP, key study, validity 2:
65% biodegradation after 28 days but failing the 10-day window.
77% biodegradation after 48 days.
Not readily biodegradable but not persistent.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable but failing 10-day window

Additional information

To assess the biodegradation potential of the registered substance, one experimental key study is available.

This study (Givaudan, 2013) was performed on the registered substance according to OECD Guideline No. 301F and EU Method C.4-D with GLP compliance. The test substance was exposed to activated sewage sludge micro-organisms at a concentration of 30 mg/L with culture medium in sealed culture vessels in the dark at 21°C for 28 days. The degradation of the test substance was assessed by the measurement of daily oxygen consumption. Control solutions with inoculum and the standard material (sodium benzoate) were used for validation purposes. However, no toxicity control was performed. Test substance undergoes 65% biodegradation after 28 days and 77% biodegradation after 48 days in the test conditions. The 10-day window criterion is not fulfilled (14% biodegradation on day 11 and 52% on day 21). Degradation of sodium benzoate exceeds 40 % after 7 days and 65 % after 14 days: the activity of the inoculum is thus verified. In conclusion, under the test conditions, test substance should be regarded as not readily biodegradable. However, based on the results the test substance should be regarded as not persistent.