Chromium trinitrate

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically valid publication in peer reviewed journal that lacks details of test conditions reporting

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)

Deviations:

not specified

GLP compliance:

no

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

chromic nitrate

IUPAC Name:

chromic nitrate

Constituent 2

Reference substance name:

Chromium trinitrate

EC Number:

236-921-1

EC Name:

Chromium trinitrate

Cas Number:

13548-38-4

Molecular formula:

Cr.3HNO3

IUPAC Name:

chromium trinitrate

Test material form:

other: aqueous solution

Details on test material:

no data reported in publication

Test animals

Species:

mouse

Strain:

Balb/c

Sex:

male

Details on test animals or test system and environmental conditions:

age of animals: 8-12 weeks

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

chromium trinitrate was provided as aqueous solution

Details on exposure:

0.5 ml applied, dose 500 Cr(NO3)3*9H2O mg/kg bw and 250 Cr(NO3)3*9H2O mg/kg bw

Duration of treatment / exposure:

single application

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
500 mg/kg bw
Basis:
analytical conc.
Cr(NO3)3 * 9H2O

Doses / concentrations 2

Remarks:

Doses / Concentrations:
250 mg/kg bw
Basis:
analytical conc.
Cr(NO3)3 * 9H2O

No. of animals per sex per dose:

5 male animals per dose were used

Control animals:

yes

Positive control(s):

no positive controls but another substance tested in this array (K2Cr2O7) was found positive.

Examinations

Tissues and cell types examined:

Erythrocytes

Details of tissue and slide preparation:

The cells of the bone marrow were extracted from the femour and suspended in serum of featal calf. After centrifugation at 1000 rpm during 8 minutes the supernatant was removed and the cells were transferred to a slide and coloured by May-Gruenwald and by Giemsa.

Evaluation criteria:

The young erythrocytes which have matured in the last 24 hours can be made visible because of their polychromatophily.

Statistics:

Method not reported in publication but statistics was applied.

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

no effects

Vehicle controls validity:

not applicable

Negative controls validity:

valid

Positive controls validity:

other: K2Cr2O7 was found positive in this test array

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative
Under the conditions of this test chromium trinitrate was found not to affect micronuclei development in vivo.

Executive summary:

In the in-vivo micronucleus test in mice reported in this publication three chromium salts were investigated for their mutagenic potential. Whereas chromium trinitrate was found to be negative, potassium dichromate (chromiumVI) was confirmed to be positive and thus can be seen as positive control in this study. In conclusion, it can be stated that chromium trinitrate was negative in the in-vivo mouse micronucleus test.