Chromium trinitrate

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in vivo (non-LLNA)

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Study period:

1967

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: CrCl3, a structural analogue of chromic nitrate, can be used for read-across.

Data source

Reference

Reference Type:

publication

Title:

Sensitization of guinea pigs to chromium salts.

Author:

Gross PR, Katz SA, Samitz MH (1968)

Year:

1968

Bibliographic source:

Journal of investigative Dermatology, 50:424–427.

Report date:

1967

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 406 (Skin Sensitisation)

Deviations:

not specified

GLP compliance:

no

Type of study:

guinea pig maximisation test

Test material

Test material information

Constituent 1

Reference substance name:

chromium chloride

IUPAC Name:

chromium chloride

Constituent 2

Reference substance name:

Chromium trichloride

EC Number:

233-038-3

EC Name:

Chromium trichloride

Cas Number:

10025-73-7

Test material form:

not specified

Details on test material:

N/A

In vivo test system

Test animals

Species:

guinea pig

Strain:

not specified

Sex:

not specified

Details on test animals and environmental conditions:

Albino guinea pigs weighing 300 and 500 Gm.

Study design: in vivo (non-LLNA)

Induction

Induction 1

Route:

other: subcutaneous

Vehicle:

no data

Induction 2

Concentration / amount:

the sensitizing injections contained 0.5 cc of Freund's complete adjuvant with 0.5 cc of 3.4 × 10-2 M CrCl3. The test dose was 0.1 cc of 4.2 × 10-4 M CrC13 in physiologic saline intradermally

Challenge

Challenge 1

Route:

intradermal

Vehicle:

no data

Challenge 2

Concentration / amount:

the sensitizing injections contained 0.5 cc of Freund's complete adjuvant with 0.5 cc of 3.4 × 10-2 M CrCl3. The test dose was 0.1 cc of 4.2 × 10-4 M CrC13 in physiologic saline intradermally

No. of animals per dose:

13: chromium chloride;
30: control group

Details on study design:

Thirteen guinea-pigs were given chromium trichloride hexahydrate by three subcutaneous injections in the nape 1 week apart. The sensitizing injections contained 0.5 ml of FCA with 0.5 ml of 3.4 × 10−2mol chromium chloride hexahydrate/l. Three weeks later, the animals were tested with an intradermal injection into clipped or epilated skin. The eliciting dose was 0.1 ml of 4.2 × 10−4 mol chromium chloride hexahydrate/l.

Challenge controls:

N/A

Positive control substance(s):

not specified

Results and discussion

Positive control results:

N/A

In vivo (non-LLNA)

Results

Reading:

1st reading

Hours after challenge:

48

Group:

test chemical

Dose level:

4.2 × 10−4 mol

No. with + reactions:

10

Total no. in group:

13

Clinical observations:

developed positive reactions (at least +) ; of these, four were read as (+2). None developed (+3) or (+4) reactions.

Remarks on result:

other: see Remark

Remarks:

Reading: 1st reading. . Hours after challenge: 48.0. Group: test group. Dose level: 4.2 × 10−4 mol . No with. + reactions: 10.0. Total no. in groups: 13.0. Clinical observations: developed positive reactions (at least +) ; of these, four were read as (+2). None developed (+3) or (+4) reactions..

Applicant's summary and conclusion

Interpretation of results:

sensitising

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Based on the results, chromium trichloride hexahydrate is a sensitizer to guinea pigs' skin at the 3.4 × 10−2 M under the conditions of this report.

Executive summary:

Thirteen guinea-pigs were given chromium trichloride hexahydrate by three subcutaneous injections in the nape 1 week apart. The sensitizing injections contained 0.5 ml of FCA with 0.5 ml of 3.4 × 10−2mol chromium chloride hexahydrate/l. Three weeks later, the animals were tested with an intradermal injection into clipped or epilated skin. The eliciting dose was 0.1 ml of 4.2 × 10-4 mol chromium chloride hexahydrate/l. After 48 h, this produced moderate positive responses in 10 of the 13 animals, whereas the control animals (injected only with FCA) showed no reactions. Of the 10 guinea-pigs sensitized to chromium chloride, 3 elicited weaker cross-reactions after intradermal injection of chromium nitrate (9.6 × 10-4 mol/l).