Registration Dossier

Administrative data

Endpoint:
additional ecotoxicological information
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Ecotoxicity studies of the levulinate ester series
Author:
Lomba L, Muñiz, S., Pino M., Navarro, E. and Giner, B.
Year:
2014
Bibliographic source:
Ecotoxicology (2014) 23:1484–1493

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: UNE-EN-ISO 11348-3 (2007) Water quality: determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test)
Type of study / information:
Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test).

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Any other information on results incl. tables

EC50 = 5687±1325 mg/l.

Applicant's summary and conclusion

Conclusions:
EC50 (30 min) = 5687±1325 mg/l.
Executive summary:

The inhibition of bioluminescence of Vibrio fischeri bacteria was assessed according to the UNE-EN-ISO 11348-3. The lyophilized V. fischeri were exposed to several dilutions for each of the chemical were prepared using a 2 % NaCl stock solution. The different concentrations ranges assessed for this bioassay were 0–20000 mg/l.

The solution equilibrated for 10 min at 15 °C, and subsequently, the first measurements were taken to obtain the initial luminescence; then 0.5 ml of each dilution to be tested was added to the cuvette. The inhibition of luminescence was measured after 30 min.

EC50 (30 min) = 5687±1325 mg/l.