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Short-term toxicity to fish

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Description of key information

The 96h-LC50 to Danio rerio (zebra fish) is 0.148 mg/L in an OECD TG 203 test under GLP.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.148 mg/L

Additional information

Seven acute toxicity tests with fish are available for various alkyl-1,3-diamines with C12-18 chains (all reliability 2) which can be used for read across to this substance N-C12 -14 -alkyltrimethylenediamines (CAS No. 90640 -43 -0). The tests reveal a comparable toxicity for the diamines with alkyl chain lengths ranging from C12 to C18, independent of the alkyl chain lenght. Justification for the read-across is provided in the attached document.

The tests were considered to be valid with restrictions for various reasons. The test concentrations were not analytically verified because a reliable specific method of analysis was not available at the time when the test was performed. Further concerns related to the poor solubility of the test substances and partial sorption onto the walls of test vessels.

The available acute fish toxicity data  are all determined with the same species, endpoint, test duration, life stage and testing conditions and they have te same reliability rating. As the results are not more than order of magnitude apart (96h LC50 range 0.08 - 0.35 mg/L),

they can be harmonised by a geometric mean according to REACH Guidance document Chapter R.7b.

The geometric mean of all 7 tests has been calculated resulting in LC50 of 0.150 mg/L. It can be concluded that fish are less sensitive to the alkyl-1,3 -diamines than daphnia and algae in acute toxicity tests based on the geometric mean of all available acute test data. Therefore it can be concluded as well that fish toxicity is not a critical endpoint for the PNEC derivation, Classification and PBT assessment. The experimental value of 0.148 mg/L is selected as key value for the CSR.

Acute toxicity to fish

CAS no

Species/test

Result

Reference

Remarks

90640-43-0

Danio rerio

0.1<96h-LC50 < 0.35 mg/L

Clariant 1985

Reliability 2, GLP

68603-64-5

Danio rerio

96h-LC50 = 0.148 mg/L

Clariant 1990

Reliability 2,

GLP

7173-62-8

Danio rerio

96h-LC50 = 0.32 mg/L

Clariant 1991

Reliability 2,

GLP

7173-62-8

Danio rerio

96h-LC50 = 0.16 mg/L

CECA 1997

 

Reliability 2,

GLP

61791-55-7

Danio rerio

96h-LC50 = 0.1 mg/L

Akzo Nobel 1990

Reliability 2,

GLP

61791-63-7

Danio rerio

96h-LC50 = 0.08 mg/L

Akzo Nobel 1993

Reliability 2,

GLP

61791-63-7

Danio rerio

96h-LC50 = 0.15 mg/L

Clariant 1990

Reliability 2, GLP