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EC number: 242-060-2
CAS number: 18172-67-3
Summary of results of the chromosomal
aberration study with Turpentine oil CAS 8006-64-2
concentration in µL/mL
in % of control
Exposure period 4 hrs without S9 mix. Experiment 1
Exposure period 22 hrs without S9 mix. Experiment 2
Exposure period 4 hrs with S9 mix Experiments 1 and 2
22 hrs, expt 1
22 hrs, expt 2
cells carrying exchanges
of 200 metaphases per culture
frequency statistically significant higher than corresponding control
0.5 % (v/v)
3 CPA 15.0
4 CPA 7.5
Test item Turpentine oil CAS
8006-64-2, dissolved in THF, was assessed for its potential to induce
structural chromosomal aberrations in human lymphocytes in vitro in
two independent experiments. The following study design was performed:
Without S9 mix
With S9 mix
Exp. I & II
In each experimental group two
parallel cultures were analysed. Per culture at least 100 metaphases
were scored for structural chromosomal aberrations.
The highest applied concentration in
the pre-test on toxicity (5.0 µL/mL of the test item) was chosen with
respect to the current OECD Guideline 473.
Dose selection of the cytogenetic
experiment was performed considering the toxicity data in accordance
with OECD Guideline 473.
In Experiment I in the absence of
S9 mix, concentrations showing clear cytotoxicity were not scorable
for cytogenetic damage. In Experiment II in the absence of S9 mix,
cytotoxicity was observed at the two highest evaluated concentrations.
In Experiment I in the presence of S9 mix, no cytotoxicity was
observed up to the highest applied concentration. In Experiment II in
the presence of S9 mix the highest applied concentration was not
evaluable due to low metaphase numbers.
In both independent experiments,
neither a statistically significant nor a biologically relevant
increase in the number of cells carrying structural chromosomal
aberrations was observed after treatment with the test item.
No evidence of an increase in
polyploid metaphases was noticed after treatment with the test item as
compared to the control cultures.
Appropriate mutagens were used as
positive controls. They induced statistically significant increases (p
< 0.05) in cells with structural chromosome aberrations.
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