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EC number: 247-426-5
CAS number: 26040-51-7
To test for its inherent biodegradability potential, the substance was
incubated for 28 days in continuously stirred 250 ml closed flask (three
replicates) in the dark with an inoculum of mixed population of aquatic
microorganisms (activated sludge) partially from domestic wastewater
treatment plant and partially from industrial origin. In this assay,
biodegradation was estimated by biological oxygen demand (BOD) over
time. BOD was determined daily measuring the drop in pressure in the
automated respirometer flasks. The incubation temperature was 25 ± 2 °C,
pH was 7.0 - 8.2. The concentration of inoculum was 100 mg /L and the
one of test substance was 30 mg/L. Degradation was calculated by
subtracting the amount BOD in the negative (inoculum only) control from
that in the test material or positive control at any given time point
and divided by the chemical oxygen demand (COD) or theoretical oxygen
demand (ThOD). The per cent degradation of the reference compound sodium
benzoate reached the level of ≥ 40 % after 7 days, but remained 2 %
below the level of ≥ 65 % after 14 days, caused by high activity of the
sludge in the blank control. As the degradation was above 40 % after 7
days (65% degradation on day 7) and the test item showed only 7 %
biodegradation after 28 days, the test was regarded to be valid. The
test item is considered to be "Not Inherently Biodegradable".
Within 28 days a degradation of 7 % was determined in a study for
biodegradability. Therefore the substance is considered to be "Not
Inherently Biodegradable". As a conclusion, the substance is considered
as not readily biodegradable as well, because there is no indication
that ready biodegradability will be observed in a ready biodegradability
test after the poor inherent biodegradability ratio of the substance in
the present study.
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