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EC number: 247-426-5
CAS number: 26040-51-7
The study was conducted in accordance with Council Regulation (EC) No
440/2008, Method C.20 'Daphnia magna Reproduction Test'', which equals
in all relevant parts to the OECD Test guideline 211 (Daphnia magna
Reproduction Test). Young female Daphnia, aged less than 24 hours at the
start of the test, were exposed to a limit test concentration of
nominally 1 mg/L of the substance dissolved in water. Auxiliaries used
to prepare the test media were an ultra turrax, a magnetic stirrer,
folded filters of pore size 7-12 μm and aseptic filters. After a
21d-exposure period, the total number of living offspring produced per
parent animal alive at the end of the test was assessed. The
reproductive output of the Daphnia exposed to the substance was compared
to that of the control in order to determine the Lowest Observed Effect
Concentration (LOEC) and the No Observed Effect Concentration (NOEC)
according to STUDENT t-Test and Fisher`s Exact Binomial Test Procedure.
Additionally, the survival of the parent Daphnia and the time to
production of first brood was determined.
21d-NOEC (reproduction): ≥ 1 mg/L
21d-LOEC (reproduction): > 1 mg/L
21d-NOEC (mortality): ≥ 1 mg/L
21d-LOEC (mortality): > 1 mg/L
No toxic effects against daphnia were observed at a limit test
concentration of nominally 1 mg/L, corresponding to a measured
concentration of < 0.0334 mg/L, which reflects the maximum water
solubility under exposure conditions.
The chronic toxicity of the substance to Daphnia magna was investigated in a semi-static test. After 21 days of exposure a NOEC(Reproduction) of >=1 mg/L (nominal) was determined. No toxic effects against daphnia were observed at a limit test concentration of nominally 1 mg/L, corresponding to a measured concentration of < 0.0334 mg/L, which reflects the maximum water solubility under exposure conditions.
"should read: NOEC >= 1 mg/L"
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