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EC number: 274-533-4 | CAS number: 70285-70-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Description of key information
The skin sensitisation classification of the target substance ADP (CAS 70285-70-0) is currently not harmonised according to the CLP Regulation (EC) 1272/2008. Consequently, based on read-across (analogue approach, see attachment "ADP_70285-70-0_Readacross_Justification” in Section 13) and the results from the source substances ATP, DI-Na (CAS 987-65-5) and AMP (CAS 61-19-8), it can be assumed that also the target substance ADP (CAS 70285-70-0) can be considered as non-sensitising to the skin (CLP not classified).
ATP, DI-Na (CAS 987-65-5)
For this endpoint three studies were considered:
QSAR DEREK NEXUS version 5.0.2 did not yield any alerts for skin sensitization for the test item. ATP, DI-Na (CAS 987-65-5) is predicted to be not sensitizing to the skin.
A valid DPRA assay was performed according to OECD 442C and GLP principles. Peptide depletion was calculated as 0.9% and 8.6% in Lysine and Cysteine Assays, respectively, resulting in a mean peptide depletion of 4.75%. This value places ATP, DI-Na (CAS 987-65-5) in the Minimal Reactivity Classification resulting in a DPRA prediction of Non-Sensitiser.
A valid Keratinosens assay was performed according to OECD 442D and GLP principles. The test item ATP, DI-Na (CAS 987-65-5) showed no toxicity (no IC30 and IC50 value). A biologically relevant, dose-related induction of the luciferase activity (EC1.5 values of 45.3 and 41.0µM in experiment 1 and 2, respectively) was measured in both experiments. The maximum luciferase activity induction (Imax) was 7.53-fold and 6.18-fold in experiment 1 and 2, respectively. ATP, Di-Na is therefore classified as positive in the KeratinoSens assay since positive results (>1.5-fold induction) were observed at test concentrations of =1000 µM with a cell viability of >70% compared to the vehicle control. However, ATP, Di-Na is considered to give false positive results in cell assays (such as the Keratinosense assay acc. to OECD 442D) because ATP is involved in cell proliferation and differentiation via the P2 receptor, which is present in most mammalian cells. Therefore, this result is considered a false positive.
In conclusion, ATP, DI-Na (CAS 987-65-5) is considered to be not sensitizing to the skin according to the results from QSAR DEREK and the DPRA assay (OECD 442C). Therefore, based on the results from non-cellular assays (namely from QSAR DEREK and the DPRA assay (OECD 442C)), ATP does not need to be classified for skin sensitization according to the CLP Regulation 1272/2008 and amendments.
AMP (CAS 61-19-8)
For this endpoint three studies were considered:
QSAR DEREK NEXUS version 5.0.2 did not yield any alerts for skin sensitization for the test item. Adenosine-5’-monophosphate (AMP) (CAS 61-19-8) is predicted to be not sensitizing to the skin.
A valid DPRA assay was performed according to OECD 442C and GLP principles. Peptide depletion was calculated as 0.0% and 1.3% in Cysteine and Lysine Assays, respectively, resulting in a mean peptide depletion of 0.65%. This value places AMP (CAS 61-19-8) in the Minimal Reactivity Classification resulting in a DPRA prediction of non-sensitiser.
A valid Keratinosens assay was performed according to OECD 442D and GLP principles. The test item showed slight toxicity in experiment 1 (IC30 value of 1441 µM and no IC50 value) and no toxicity in experiment 2. A biologically relevant, dose-related induction of the luciferase activity (EC1.5 values of 22.8 and 25.8µM in experiment 1 and 2, respectively) was measured in both experiments. The maximum luciferase activity induction (Imax) was 21.9-fold and 13.6-fold in experiment 1 and 2, respectively. AMP is classified as positive in the KeratinoSens assay since positive results (>1.5-fold induction) were observed at test concentrations of <1000 µM with a cell viability of >70% compared to the vehicle control. However, AMP is considered to give false positive results in cell assays (such as the Keratinosense assay acc. to OECD 442D), because interconversion of AMP to ATP could be expected as result of normal metabolic activity of the cells during the experiment, and because ATP is involved in cell proliferation and differentiation via the P2 receptor, which is present in most mammalian cells. Therefore, this result is considered a false positive.
In conclusion, AMP (CAS 61-19-8) is considered to be not sensitising to the skin according to the results from QSAR DEREK and the DPRA assay (OECD 442C). Therefore, based on the results from non-cellular assays (namely from QSAR DEREK and the DPRA assay (OECD 442C)), AMP does not need to be classified for sensitization according to the CLP Regulation 1272/2008 and amendments.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- See attachment "ADP_70285-70-0_Readacross_Justification" in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- other: alerts for skin sensitization
- Remarks on result:
- other: DEREK NEXUS version 5.0.2 did not yield any alerts for skin sensitization for the test item. ATP, Di-Na is predicted to be not sensitizing to the skin.
- Interpretation of results:
- other: Non-Sensitizer
- Conclusions:
- DEREK NEXUS version 5.0.2 did not yield any alerts for skin sensitization for the test item. ATP, Di-Na (CAS Nr. 987-65-5) is predicted to be not sensitizing to the skin.
Based on the readacross, it can be assumed that also the target substance ADP (CAS 70285-70-0) is predicted as not sensitizing to the skin. - Endpoint:
- skin sensitisation: in vitro
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- See attachment "ADP_70285-70-0_Readacross_Justification" in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Run / experiment:
- other: 1
- Parameter:
- Imax [442D]
- Remarks:
- maximal average fold induction of luciferase activity (Imax)
- Value:
- 7.53
- Positive controls validity:
- valid
- Remarks:
- Imax: 2.69
- Remarks on result:
- other: maximal average fold induction of luciferase activity (Imax):7.53 fold
- Key result
- Run / experiment:
- other: 2
- Parameter:
- Imax [442D]
- Value:
- 6.18
- Positive controls validity:
- valid
- Remarks:
- Imax: 4.23
- Remarks on result:
- other: maximal average fold induction of luciferase activity (Imax): 6.18 fold
- Key result
- Run / experiment:
- other: 1
- Parameter:
- EC 1.5 [442D]
- Value:
- 45.3 µM
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks:
- EC1.5: 13.7 µM
- Key result
- Run / experiment:
- other: 2
- Parameter:
- EC 1.5 [442D]
- Value:
- 41 µM
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks:
- EC1.5 46.0 µM
- Interpretation of results:
- other: activation of the antioxidant/electrophile responsive element (ARE)-dependent pathway in keratinocytes
- Conclusions:
- In conclusion, ATP, Di-Na (CAS No 987-65-5) is classified as positive (activation of the antioxidant/electrophile responsive element (ARE)-dependent pathway in keratinocytes) under the experimental conditions described in this report.
Based on readacross, also the target substance ADP (CAS 70285-70-0) is predicted to be classified as positive (activation of the antioxidant/electrophile responsive element (ARE)-dependent pathway in keratinocytes). - Endpoint:
- skin sensitisation: in chemico
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- See attachment "ADP_70285-70-0_Readacross_Justification" in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Run / experiment:
- mean
- Parameter:
- other: Mean depletion value
- Value:
- 4.75 %
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks:
- Cinnamic aldehyde
- Remarks on result:
- other: Minimal reactivity (non sensitizer)
- Remarks:
- Minimal reactivity (non Sensitizer)
- Interpretation of results:
- other: minimally reactive: non-sensitizer
- Conclusions:
- In conclusion, according to the DPRA cysteine and lysine prediction model, ATP, Di-Na (CAS 987-65-5) was classified as minimally reactive and was, therefore, a non-sensitiser.
Based on readacross, also the target substance ADP (CAS 70285-70-0) is predicted as minimally reactive and, therefore, as non-sensitiser. - Endpoint:
- skin sensitisation: in vitro
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- See attachment "ADP_70285-70-0_Readacross_Justification" in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Run / experiment:
- other: 1
- Parameter:
- Imax [442D]
- Value:
- 21.9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks:
- Imax: 3.40
- Remarks on result:
- other: Imax: 21.9
- Key result
- Run / experiment:
- other: 2
- Parameter:
- Imax [442D]
- Value:
- 13.64
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks:
- Imax: 13.62
- Remarks on result:
- other: Imax: 13.64
- Key result
- Run / experiment:
- other: 1
- Parameter:
- EC 1.5 [442D]
- Value:
- 22.8 µM
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks:
- EC1.5: 24.4 µM
- Key result
- Run / experiment:
- other: 2
- Parameter:
- EC 1.5 [442D]
- Value:
- 25.8 µM
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks:
- EC1.5 15.8 µM
- Interpretation of results:
- other: activation of the antioxidant/electrophile responsive element (ARE)-dependent pathway in keratinocytes
- Conclusions:
- In conclusion, the KeratinoSensTM assay is valid and Adenosine-5’-monophosphate (AMP) (CAS no 61-19-8) is classified as positive (activation of the antioxidant/electrophile responsive element (ARE)-dependent pathway in keratinocytes) under the experimental conditions used in this study.
Based on readacross, also the target substance ADP (CAS 70285-70-0) is predicted to be classified as positive (activation of the antioxidant/electrophile responsive element (ARE)-dependent pathway in keratinocytes). - Endpoint:
- skin sensitisation: in chemico
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- See attachment "ADP_70285-70-0_Readacross_Justification" in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Run / experiment:
- mean
- Parameter:
- other: Mean depletion value
- Value:
- 0.65 %
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks:
- Cinnamic aldehyde
- Remarks on result:
- other:
- Remarks:
- Minimal reactivity (Non-sensitizer)
- Interpretation of results:
- other: minimally reactive: non-sensitizer
- Conclusions:
- In conclusion, according to the DPRA cysteine and lysine prediction model, AMP (CAS no. 61-19-8) was classified as minimally reactive and was, therefore, a non-sensitiser.
Based on readacross, also the target substance ADP (CAS 70285-70-0) is predicted as minimally reactive and, therefore, as non-sensitiser. - Endpoint:
- skin sensitisation, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- See attachment "ADP_70285-70-0_Readacross_Justification" in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Parameter:
- other: alerts for skin sensitization
- Remarks on result:
- other: DEREK NEXUS version 5.0.2 did not yield any alerts for skin sensitization for the test item. Adenosine 5’-monophosphate (AMP) is predicted to be not sensitizing to the skin.
- Interpretation of results:
- other: Non-Sensitizer
- Conclusions:
- DEREK NEXUS version 5.0.2 did not yield any alerts for skin sensitization for the test item. Adenosine-5’-monophosphate (AMP) (CAS Nr. 61-19-8) is predicted to be not sensitizing to the skin.
Based on readacross, it can be assumed that also the target substance ADP (CAS 70285-70-0) is predicted as not sensitizing to the skin.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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