Registration Dossier
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EC number: 204-815-4 | CAS number: 126-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 29/06/03 to 09/02/05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Dept. of Health, UK
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not relevant - Analytical monitoring:
- yes
- Details on sampling:
- The test samples were analysed following filtration through glass wool to remove algal cells.
- Vehicle:
- no
- Details on test solutions:
- For the purpose of the definitive test, the test material was dissolved directly in culture medium.
An amount of test material (195.5 mg) was dissolved in culture medium and the volume adjusted to 0,5 L to give a 200 mg ai/L stock solution from which serial delutions were made to give 100, 50, 25, 12.5 mg ai/L stock solutions. An aliquot (250 mL) of each stock solution was mixed with algal suspension (250 mL) to give the required test concentrations of 6.25, 12,5, 25, 50 and 100 mg ai/L. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- - Strain: CCAP 276/20
- Source: Culture Collection of Algae and Protozoa (CCAP), Institute of Freshwater Ecology, Cumbria.
- The culture was maintained in the laboratory at a temperature of 21°C (+ or - 1°C), under continuous illumination (intensity approximately 7000 lux) and constant aeration. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- no data
- Test temperature:
- 21 °C (+ or - 1°C)
- pH:
- From pH 7.3 at 0 hours to pH 8.3 at 72 hours
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml glass conical flasks.
- Flasks plugged with polyurethane foam bungs
- 3 flasks each containing 100 ml of test preparation were used for the control and each treatment group.
- Initial nominal cell density: 10^4 cells per mL
OTHER TEST CONDITIONS
- Illumination: continuous (intensity approximately 7000 lux)
- Shaking: constant, at 150 rpm for 72 hours.
TEST CONCENTRATIONS
- Range finding study:
- Test concentrations: nominal test concentrations of 0.10, 1.0, 10 and 100 mg ai/l.
- Results used to determine the conditions for the definitive study: After 72 hours no effect on algal growth was observed. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% confidence limits: 34 - 45 mg ai/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 65 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limits 58 - 74 mg ai/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: There were no statistically significant differences between the control, the 6.25 and the 12.5 mg ai/L test concentrations (P≥0.05).
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% confidence limits 2.4 – 2.8 mg ai/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limits 3.0 – 3.5 mg ai/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.45 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- The test material was unstable in the test medium for the duration of the test.
- Results with reference substance (positive control):
- no data
- Validity criteria fulfilled:
- yes
- Remarks:
- The cell concentration of the control cultures increased by a factor of 49 after 72 hours. This increase was in line with the OECD Guideline that states the enhancement must be at least by a factor of 16 after 72 hours.
- Conclusions:
- Given this decline in measured test concentrations it was considered justifiable to base the results on the nominal test concentrations (ai: active ingredient) of the test item itself. The 72h-EC50 (biomass) based on the nominal test concentrations was 39 mg ai/L and the 72h- EC50 (growth rate) was 65 mg ai/L. The 72h No Observed Effect Concentration (NOEC) was 1.45 mg ai/L.
Reference
Description of key information
The EC50 (72 h) for freshwater algae (Desmodesmus subspicatus): 39 mg/L (biomass; OECD TG 201; static).
NOEC (72h): 12.5 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 39 mg/L
- EC10 or NOEC for freshwater algae:
- 12.5 mg/L
Additional information
It was considered justifiable to base the results on the nominal test concentrations of MeaTG, despite the fact that the test material was slightly unstable in the test medium for the duration of the test.
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