(2-hydroxyethyl)ammonium mercaptoacetate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals

Author:

Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K and Speck W

Year:

1987

Bibliographic source:

Environ Mutagen, 9(suppl.9), 1-109

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Mercaptoacetic acid

EC Number:

200-677-4

EC Name:

Mercaptoacetic acid

Cas Number:

68-11-1

Molecular formula:

C2H4O2S

IUPAC Name:

sulfanylacetic acid

Details on test material:

Test compound: Thioglycolic acid
CAS no.: 68-11-1
Source: Aldrich
Batch: no data
Purity: 98%

Method

Target gene:

Histidine reversion

Species / strain

Species / strain / cell type:

S. typhimurium, other: Strains: TA97, TA98, TA100 & TA1535

Metabolic activation:

with and without

Metabolic activation system:

10% and 30 of Rat and Hamster liver S9 induced with Aroclor 1234

Test concentrations with justification for top dose:

0, 33, 100, 333, 1000, 2000, 3333 µg/plate

Vehicle / solvent:

water

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: Without activation : sodium azide (TA1535 and TA 100),    9-aminoacridine (TA 97), 4-nitro-o-phenylenediamine (TA98).  With activation : 2-aminoanthracene (all strains).

Evaluation criteria:

The positive control plates are counted, and the number of mutant  colonies appearing on them must be significantly increased over the  spontaneous control number for the test to be considered valid. 
If no increase in mutant colonies is seen after testing several strains  under several different culture conditions, the test chemical is  considered to be non-mutagenic in the Salmonella test.

Statistics:

None

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 97

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid

Test results 3

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid

Test results 4

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

Chemical Name:	Thioglycolic acid
CAS Number:	68-11-1
Study Type:	Salmonella
Study ID:	301735
Study Result:	Negative
Year Completed:	1983
Vehicle Control:	Water
Protocol:	Preincubation

Table Instructions and Notes: You are currently viewing the results for this Salmonella study's detailed data.
Individual strain data is presented as mean ± standard error.
Abbreviations are noted at bottom of page.
Trial summary calls are shown in parentheses.
Strain: TA1535
Dose	No Activation		No Activation		10% HLI		30% HLI		10% RLI		30% RLI
	(Negative)		(Negative)		(Negative)		(Negative)		(Negative)		(Negative)
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	30	4.9	43	3.4	11	1.2	15	3.2	13	1.5	17	2.6
33	26	3.2	38	7.8	7	1.5	15	1.5	7	0.3	14	0
100	28	4.2	41	1.2	9	1.5	16	1.7	11	1.5	14	3.1
333	23	1.8	41	4.3	9	2.3	12	2.3	9	0.3	14	2.5
1000	9	4.9	28	5	7	1.2	11	2	9	0.9	11	0.9
2000					10	1.2	10s	1.2	6	2	10s	2
3333	1s	0.3	13s	1.5
Positive Control	1065	17.4	531	10.7	122	11.5	154	5.9	89	2	121	8.4
Strain: TA100
Dose	No Activation		No Activation		10% HLI		30% HLI		10% RLI		30% RLI
	(Negative)		(Negative)		(Negative)		(Negative)		(Negative)		(Negative)
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	91	3.2	129	5	83	9	109	4.7	100	3.7	133	8.7
33	91	10.1	108	9.9	85	1.5	111	7.9	90	7.2	103	2.2
100	92	6.7	113	9.2	77	2.5	100	11.9	94	7.2	127	6.4
333	81	1.3	113	6.9	101	3.3	89	4.4	107	9.3	101	8.1
1000	78	2.3	88	4.7	87	7.5	104	1.7	89	3	123	7.5
2000					63	9.6	88s	8.3	63	5.8	90s	3
3333	2s	0.9	39s	6.3
Positive Control	1407	48.6	1122	27.6	1373	25.2	400	39.4	1218	11.7	416	10.4

Applicant's summary and conclusion

Conclusions:

TGA was negative in the Ames test, without and with metabolic activation