(2-hydroxyethyl)ammonium mercaptoacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997-02-04 - 1997-04-14

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Dept. of Environment, Lower Saxony, Germany

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

(2-hydroxyethyl)ammonium mercaptoacetate

EC Number:

204-815-4

EC Name:

(2-hydroxyethyl)ammonium mercaptoacetate

Cas Number:

126-97-6

Molecular formula:

C2H7NO.C2H4O2S

IUPAC Name:

2-hydroxyethan-1-aminium 2-sulfanylacetate

Constituent 2

Reference substance name:

MEATG

IUPAC Name:

MEATG

Test material form:

solid - liquid: suspension

Details on test material:

Batch number: B97S1957c
Content: 67% MEATG

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Source: Fa. Harlan Winkelmann GmbH, 33178 Borchen, Germany
Strain: Hsd/Dpb:WU
Weight range at study initiation: 213-298g (m), 179-205g (f)
Housing: collective housing up to a maximum of 3 animals per cage (Makrolon type III)Illumination: artificial lighting from 7.00 a.m.-7.00 p.m.
Temperature: 22±3 °C
Relative humidity: 30-70

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

140, 168, 202, 242, 290 and 348mg/kg of the test substance.

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Clinical observations were conducted at regular intervals during the 14-day observation period.
Gross pathological examinations were performed immediately on animals found dead and at termination on day 14 on surviving animals.
Body weights were measured at days 0, 7 and 14.

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

318 mg/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

181 mg/kg bw

Based on:

test mat.

Mortality:

19 of 42 animals died pre-terminally.

Clinical signs:

other: Severe dose-dependent clinical signs were observed. The most striking findings were reduced activity, squatting position, abnormal galt and abnormal body posture, decreased body tone and decreased respiratory rate.

Gross pathology:

Gross pathological examinations on day 14 post administration (terminal necropsy) revealed no test article-dependent findings. However, animals found dead showed alterations, which were considered to be test article-related.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

MeaTG can be classified as toxic (Acute Tox 3).