Phenyl chloroformate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Predates implementation of GLP and/or development of study guidelines, restrictions in reporting and design. It is stated that the test substance is insoluble in water. However, no analytical monitoring has been performed. Therefore it is unknown what the actual concentration of the test substance is.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Principles of method if other than guideline:

Acute fish toxicity test in which 10 Gold Ide per concentration (0, 8, 10, 12.5, 16.0, 20.0 and 100 mg/L) were exposed to the test substance for 96 hours.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Phenyl chloroformate

EC Number:

217-547-8

EC Name:

Phenyl chloroformate

Cas Number:

1885-14-9

Molecular formula:

C7H5ClO2

IUPAC Name:

phenyl chloroformate

Test material form:

other: colorless liquid

Details on test material:

- Name of test material (as cited in study report): Chlorameisensäurephenylester
- Physical state: colorless liquid
- Purity: Chemically pure

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance is insoluble in water. The test substance is suspended in the experimental water using an Ultra-Turrax for 5 minutes at the highest rotation speed, transferred to the test tanks and evenly distributed with a glass rod. The stated concentrations are therefore not dissolved fractions but the total applied amount of the test substance in the tanks.

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: Gold Ide (Goldorfe)
- Source: Paul Eggers, 2345 Hohenwestedt
- Length at study initiation (length definition range (mean, SD): 5.5 - 6.5 cm (6.0, 0.34)
- Weight at study initiation (range (mean, SD): 1.7 - 2.6g (2.1, 0.27)
- Food type: Tetra Min (Tetra Werke, Melle)

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): in experimental water at 20°C.

About 65 hours prior to treatment 10 fish per tank were installed.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness was 9.4°d and the carbonate hardness was 6.5°d.

Test temperature:

20 ± 1°C

pH:

8.1 - 8.3.

Dissolved oxygen:

>8mg/L

Nominal and measured concentrations:

Nominal: 0, 8, 10, 12.5, 16.0, 20.0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass tanks
- Material, size, headspace, fill volume: glass tanks, 40 x 25 x 30cm, 20L
- Aeration: Tanks were ventillated during total testperiod over glass capillaries. About 10 0mL air per minute was passed though the tanks.
- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Deionised tap water with a water conductance < 5µS/cm. To this water following salts were added according to the formulation of Marking and Dawson: 192 mg NaHCO3; 120 mg CaSO4.H2O; 120 mg MgSO4 and 8 mg KCl.
- Intervals of water quality measurement: directly before test material application, and after 2, 24, 48, 72 and 96 hours, pH, O2 and temperature were measured per tank.

OTHER TEST CONDITIONS
- Photoperiod: Day/Night rythm, 12 hours light from above
- Light intensity: 700 Lux

EFFECT PARAMETERS MEASURED:
-Symptoms, behaviour, mortality, macroscopic examination

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

10.6 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Remarks on result:

other: CL (95%): 9.21 - 12.0

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10.6 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Remarks on result:

other: CL (95%): 9.21 - 12.0

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Details on results:

- Mortality rate: See any other information on results incl. tables.
- The fish died between 18 minutes and 23 hours after substance application. The following symptoms were recorded: uncoordinated swimming movements, hyper reflexia, escape attempts, back or side position, reddish external discoloration, strong perfused fin base. Behaviour in the 10 and 8 mg/L group was normal again after 48 hours.
- Section of the deceased and killed fish at the end of treatment revealed no macroscopically visible changes in all experimental groups.
- The pH was in the 100 mg/L group decreased to 6.7 two hours after substance addition. In all other experimental groups the pH (7.8 to 8.3) as well as the measured oxygen concentration (8.1 - 9.0 mg/L) were in the region of the measured values of the control group.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Concentration (mg/L)	Mortality
	48 Hours		96 Hours
	Absolute	Relative (%)	Absolute	Relative (%)
0	0/20	0	0/20	0
8	2/10	20	2/10	20
10	2/10	20	2/10	20
12.5	9/10	90	9/10	90
16	9/10	90	9/10	90
20	10/10	100	10/10	100
100	10/10	100	10/10	100

Applicant's summary and conclusion

Conclusions:

The LC50 of Phenyl chloroformate tested with Gold Ide was 10.6 mg/L after 48 and 96 hours in this study.

Executive summary:

The acute toxicity of phenyl chloroformate to fish was tested. Ten Gold Ide per concentration (0, 8, 10, 12.5, 16.0, 20.0 and 100 mg/L) were exposed to the test substance for 96 hours. After 48 and 96 hours, a LC50 of 10.6 mg/L was observed. However, since the test substance is not soluble in water and no analytical monitoring has been performed, the actual concentration to which the fish are exposed are unknown.