Registration Dossier

Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Toxicity of metals to tadpoles of the common Sunda toad, Duttaphrynus melanostictus
Author:
Shuhaimi-Othman M, Nadzifah Y, Umirah NS, Ahmad AK
Year:
2012
Bibliographic source:
Toxicological and Environmental Chemistry 94(2):364-376
Reference Type:
publication
Title:
Deriving freshwater quality criteria for iron, lead, nickel, and zinc for protection of aquatic life in Malaysia
Author:
Shuhaimi-Othman M, Nadzifah Y, Nur-Amalina R, Umirah NS
Year:
2012
Bibliographic source:
The Scientific World Journal, 7p

Materials and methods

GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- N2O6Pb
- purity: 99.6 %
- analytical grade
- lot 086196

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
samples taken before and after each solution renewal; acidified to 1% with ARISTAR nitric acid

Test solutions

Details on test solutions:
dechlorinated tap water

Test organisms

Aquatic vertebrate type (other than fish):
toad
Test organisms (species):
other: Duttaphrynus melanostictus
Details on test organisms:
fourth instar ; 0.11 g ; 2.0-2.5 cm

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
18.6 mg/L as CaCO3
Test temperature:
28-30 °C
pH:
6.5
Dissolved oxygen:
6.3 mg/L
Nominal and measured concentrations:
not specified
Details on test conditions:
10 organisms per treatment; 2 replicates per treatment
5 organisms per replicate
no feeding during the experiment
12/12 L:D photoperiod

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 500 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element (total fraction)
Basis for effect:
mortality
Remarks on result:
other: 95%CL: 400-2200 µg Pb/L
Reported statistics and error estimates:
LC50 was calculated using measured metal concentrations. FORTRAN programmes based on the methods of Litchfield (1949) and Litchfield and Wilcoxon (1949) were used to compute the LC50s.

Applicant's summary and conclusion

Conclusions:
A 96h-LC50 of 1500 µg Pb/L was determined for the tadpole Duttaphrynus melanostictus and can be considered in a weight-of-evidence approach for the hazard and risk assessment of Pb.