Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report Date:
1983

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: EPA-660/3-75-009, "Methods for acute toxicity with fish, macrovertebrates, and amphibians"
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): OS #26953F
- Substance type: organic
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution
- Chemical name of vehicle: water

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: laboratory in house culture
- Age at study initiation: juvenile daphnids, less than 24-hours old
- Feeding during test: no feeding1

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
160 - 180 mg/L as CaCO3
Test temperature:
20°C +/- 1
pH:
7.9 - 8.3
Dissolved oxygen:
7.9 - 9.5 mg 02/mL
Nominal and measured concentrations:
nominal concentrations:
control; 78; 130; 220; 360; 600 and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: beaker containing each 200 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water was fortified according to the test protocol and filtered through a carbon filter and an Amberlite ZAD-7 resin column to remone any potential organic contaminants
- Alkalinity: 110- 130 mg/L
- Conductivity: 400 - 600 microhos/cm

OTHER TEST CONDITIONS
- Photoperiod: 16 hour-light and 8-hour darkness

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6 - 1.7
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
340 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: CI 95% 280 - 340
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Other biological observations: control response was satisfactory (100% survival and no sublethal effects)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for acute toxicty at 48 hours on Daphnia magna of ATBS was shown to be 340 mg/mL (CI95% 280 - 430 mg/L). The No Observed Effect Concentration (NOEC) was 78 mg/l at 48 hours.