2-acrylamido-2-methylpropanesulphonic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009, "Methods for acute toxicity with fish, macrovertebrates, and amphibians"

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-acrylamido-2-methylpropanesulphonic acid

EC Number:

239-268-0

EC Name:

2-acrylamido-2-methylpropanesulphonic acid

Cas Number:

15214-89-8

Molecular formula:

C7H13NO4S

IUPAC Name:

(1Z)-N-(2-methyl-1-sulfopropan-2-yl)prop-2-enimidic acid

Details on test material:

- Name of test material (as cited in study report): OS #26953F
- Substance type: organic
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution
- Chemical name of vehicle: water

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: laboratory in house culture
- Age at study initiation: juvenile daphnids, less than 24-hours old
- Feeding during test: no feeding1

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

160 - 180 mg/L as CaCO3

Test temperature:

20°C +/- 1

pH:

7.9 - 8.3

Dissolved oxygen:

7.9 - 9.5 mg 02/mL

Nominal and measured concentrations:

nominal concentrations:
control; 78; 130; 220; 360; 600 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: beaker containing each 200 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water was fortified according to the test protocol and filtered through a carbon filter and an Amberlite ZAD-7 resin column to remone any potential organic contaminants
- Alkalinity: 110- 130 mg/L
- Conductivity: 400 - 600 microhos/cm

OTHER TEST CONDITIONS
- Photoperiod: 16 hour-light and 8-hour darkness

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6 - 1.7

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

340 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: CI 95% 280 - 340

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

78 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Other biological observations: control response was satisfactory (100% survival and no sublethal effects)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for acute toxicty at 48 hours on Daphnia magna of ATBS was shown to be 340 mg/mL (CI95% 280 - 430 mg/L). The No Observed Effect Concentration (NOEC) was 78 mg/l at 48 hours.