Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report Date:
1983

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: EPA-66013-75-009, "methods for acute toxicity tests with fish, macrovertebrates, and amphibians.
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): OS #26935F
- Substance type: organic
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution
- Chemical name of vehicle: soft water reconstituted from deionized water
- stock solution: 150 mg/mL
- final test solutions: 130, 220, 360, 600 and 1000 mg/L

Test organisms

Test organisms (species):
Lepomis macrochirus
Details on test organisms:
TEST ORGANISM
- Common name: Bluegill
- Source: in house laboratory supply (Lot # 83A13)
- Age at study initiation: juvenile
- Length at study initiation: 3.8 cm
- Weight at study initiation: 0.66 g (wet weight)
- Feeding during test: no feeding during the study

ACCLIMATION
- Acclimation period: 14 days prior to start the test
- Acclimation conditions: same as test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
44 mg/L as CaCO3
Test temperature:
22°C +/- 1
pH:
7.5
Dissolved oxygen:
5.5 to 9.2 mg 02/L
Nominal and measured concentrations:
Nominal concentration:
Control, 130; 220; 360; 600 andd 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: glass exposure vessels containing 15 L of test solution
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): no replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: soft water reconstituted from dionized water
- Alkalinity: 32 mg/L
- Conductivity: 140 microhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours darkness
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6 - 1.7
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
130 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
170 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 130 - 220
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
170 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 130 - 220
Details on results:
- Abnormal responses: sublethal effects including loss of equilibrium and rapid respiration was seen at 0 hours in the 600 and 1000 mg/L test concentration

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC0 at 96 hours for fish of ATBS was 130 mg/L and the LC50 at both 72 and 96 hours was calculated as 170 mg/L.