Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1986
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986
Report Date:
1986

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: 40 CFR 795.3340
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): OS6134?E
- Molecular formula (if other than submission substance): C7H13NO4S.Na
- Molecular weight (if other than submission substance): 229.23
- Smiles notation (if other than submission substance): [Na+].CC(C)(CS(=O)(=O)[O-])NC(=O)C=C
- InChl (if other than submission substance): InChI=1/C7H13NO4S.Na/c1-4-6(9)8-7(2,3)5-13(10,11)12;/h4H,1,5H2,2-3H3,(H,8,9)(H,10,11,12);/q;+1/p-1
- Substance type: acrylamide
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: ambient temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge : O'Bannon Creek domestic wastewater treatment plant
- Laboratory culture: raw sewage collected from a primary settling tank was used as the influent during acclimation period and test periods;
- Preparation of inoculum for exposure: At the end of the acclimation period, sludge from all units was pooled and mixed. Adequate amount was added to each unit to achieve a suspended solids concentration of 1500 mg/L for each unit. One mg carbon/100 ml influent was added to the appropriate test units.
- Pretreatment: The acclimation process included aeration for 23 hours, settling of sludge, removing 100 ml of supernatant and resuming aeration to 500 mg/minute. The units were fed raw sewage daily till a clear supernatant liquor was obtained.
Concentration of test chemical: From day 1 to day 8, 20 mg carbon/l was introduced into the test unit. This was reduced to 10 mg carbon/l from day 8 on. No organic solvents were used to facilitate the dispersion of the test material. The test substance was weighed onto a teflon coupon and introduced into the medium.
Duration of test (contact time):
44 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Solubilising agent: no
- Aeration of dilution water: 500 mg/minute
- Suspended solids concentration: 1500 mg/L for each unit
TEST SYSTEM
- Culturing apparatus: aeration units consisting of glass vessels with cone shaped lower ends. Units contained 150 ml of mixed liquor when filled
SAMPLING
- Sampling frequency: collected all workdays
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
< 10
Sampling time:
44 d
Details on results:
Thes study was extended to 44 days to determine if acclimation and biodegradation would increase in this time frame.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: <10% after 44 days
Conclusions:
The test substance showed a low biodegradation rate of less than 10% after 44 days. ATBS was not considered to be readily biodegradable.