2-acrylamido-2-methylpropanesulphonic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 40 CFR 795.3340

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate

EC Number:

225-948-4

EC Name:

Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate

Cas Number:

5165-97-9

IUPAC Name:

sodium 2-(acryloylamino)-2-methylpropane-1-sulfonate

Details on test material:

- Name of test material (as cited in study report): OS6134?E
- Molecular formula (if other than submission substance): C7H13NO4S.Na
- Molecular weight (if other than submission substance): 229.23
- Smiles notation (if other than submission substance): [Na+].CC(C)(CS(=O)(=O)[O-])NC(=O)C=C
- InChl (if other than submission substance): InChI=1/C7H13NO4S.Na/c1-4-6(9)8-7(2,3)5-13(10,11)12;/h4H,1,5H2,2-3H3,(H,8,9)(H,10,11,12);/q;+1/p-1
- Substance type: acrylamide
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: ambient temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge : O'Bannon Creek domestic wastewater treatment plant
- Laboratory culture: raw sewage collected from a primary settling tank was used as the influent during acclimation period and test periods;
- Preparation of inoculum for exposure: At the end of the acclimation period, sludge from all units was pooled and mixed. Adequate amount was added to each unit to achieve a suspended solids concentration of 1500 mg/L for each unit. One mg carbon/100 ml influent was added to the appropriate test units.
- Pretreatment: The acclimation process included aeration for 23 hours, settling of sludge, removing 100 ml of supernatant and resuming aeration to 500 mg/minute. The units were fed raw sewage daily till a clear supernatant liquor was obtained.
Concentration of test chemical: From day 1 to day 8, 20 mg carbon/l was introduced into the test unit. This was reduced to 10 mg carbon/l from day 8 on. No organic solvents were used to facilitate the dispersion of the test material. The test substance was weighed onto a teflon coupon and introduced into the medium.

Duration of test (contact time):

44 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Solubilising agent: no
- Aeration of dilution water: 500 mg/minute
- Suspended solids concentration: 1500 mg/L for each unit
TEST SYSTEM
- Culturing apparatus: aeration units consisting of glass vessels with cone shaped lower ends. Units contained 150 ml of mixed liquor when filled
SAMPLING
- Sampling frequency: collected all workdays
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

< 10

Sampling time:

44 d

Details on results:

Thes study was extended to 44 days to determine if acclimation and biodegradation would increase in this time frame.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: <10% after 44 days

Conclusions:

The test substance showed a low biodegradation rate of less than 10% after 44 days. ATBS was not considered to be readily biodegradable.