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EC number: 204-881-4
CAS number: 128-37-0
A study to assess the toxicity of BHT to the aquatic protozoa Tetrahymena
pyriformis was conducted. The experimental animals were pre-cultured
at 30 ºC for 24 h. The stock solution of the chemical was added to the
sterile medium to provide a concentration ratio of 1.8 in 10 mL of 2%
proteose peptone. The solutions were then inoculated with 0.2 ml of
pre-cultured T. pyriformis and cultivated at 30 ºC for 24 h without
agitation. The number of cells was then counted. The relative growth
rate was calculated as the ratio of the number of cells cultured wth a
chemical against the number cultivated in a blank. The effective
concentration 50% (EC50) values were obtained by plotting the relative
growth rates against the concentration of chemical on
logarithmic-probability paper. The 24 -hour EC50 for BHT was 1.7 mg/L.
Tetrahymena pyriformis. EC50 (24h)= 1.7 mg/L based on growth inhibition.
The 30min-EC50 of BHT to Photobacterium phosphoreum based on light
emission is 8.98 mg/L.
The 36h-NOEC of BHT to Pseudomona fluorescens based on the number of
viable cells was 50 mg/L.
Weight of evidence
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