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EC number: -
CAS number: -
The objective of this study was to determine the acute toxicity of
MEAHA-Fe to the earthworm Eisenia foetida (Savigny 1826). A
range-finding test was carried out to determine the concentrations of
the test substance that caused 100% and 0% mortality. According to the
results obtained in the range-finding test, a definitive test was
carried out with the following treatments: 0 (water control) and 1000
mg/kg of artificial soil. Forty earthworms per treatment were used in
the definitive test, distributed into four replicates of 10 earthworms
each. The test was carried out in polyethylene containers placed inside
a room with continuous light for fourteen days. Mortality was determined
at 7 and 14 days of incubation. Temperature and humidity were both
measured throughout the test and were within acceptable limits. The
control treatment did not cause any mortality to the earthworms during
14 days exposure period.
The highest tested concentration (1000 mg/kg of artificial soil) of test
substance did not cause mortality to the test organisms. Therefore, the
14-days LC50 value, of the acute toxicity test, for earthworm E. foetida
exposed to tested substance was considered greater than 1000 mg/kg of
standard OECD 207 test, the 14-days LC50,
of the acute toxicity test, for earthworm Eisenia
to test substance MEAHA-Fe was considered higher than 1000 mg/kg of
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