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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reason / purpose:
read-across source
Reason / purpose:
read-across: supporting information
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
In conclusion the 96 hour LC50 value for FeNa-EDDHA with the zebrafish (Danio rerio) was > 120 mg/L based on the nominal concentration of the test substance and > 122 mg/L based on the mean of analytically determined concentrations. The 96 hour NOEC was determined to be greater than or equal to 120 mg/L.

Based on the structural similarity, the similar physico-chemical properties and the similar toxicity to aquatic invertebrates and algae between FeNa-EDDHA and MEAHA-Fe, identical conclusions are drawn for the read-across target MEAHA-Fe.
Executive summary:

The acute toxic effects (lethality) of FeNa-EDDHA to zebrafish over a 96 hour static exposure period were determined according to the principles of OECD-Guideline 203 and EU method C.1 (limit test). The test substance was tested at 120 mg/L, which was analytically confirmed.

The 96 hour LC50 value for FeNaEDDHA with the zebrafish (Danio rerio) was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean of analytically determined concentrations. The 96 hour NOEC was determined to be greater than or equal to 120 mg/L. Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20% of the nominal concentrations, the effect concentration is expressed relative to the nominal concentration.

Based on the structural similarity, the similar physico-chemical properties and the similar toxicity to aquatic invertebrates and algae between FeNa-EDDHA and MEAHA-Fe, identical conclusions are drawn for the read-across target MEAHA-Fe.

Description of key information

The acute toxic effects (lethality) of FeNaEDDHA to zebrafish over a 96 hour static exposure period were determined according to the principles of OECD-Guideline 203 and EU method C.1 (limit test). The test substance was tested at 120 mg/L, which was analytically confirmed.

The 96 hour LC50 value for FeNaEDDHA with the zebrafish (Danio rerio) was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean of analytically determined concentrations. The 96 hour NOEC was determined to be greater than or equal to 120 mg/L. Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20% of the nominal concentrations, the effect concentration is expressed relative to the nominal concentration.

Based on the structural similarity, the similar physico-chemical properties and the similar toxicity to aquatic invertebrates and algae between FeNa-EDDHA and MEAHA-Fe, identical conclusions are drawn for the read-across target MEAHA-Fe.

Key value for chemical safety assessment

LC50 for freshwater fish:
120 mg/L

Additional information

In the key study the acute toxic effects of FeNa-EDDHA to zebrafish were determined according to the principles of OECD-Guideline 203 and EU method C.1. A limit test with a nominal concentration of 120 mg/L was carried out in a static system. The LC50 for a 96 hour exposure was greater than 120 mg/L and the NOEC was greater than or equal to 120 mg/L for zebrafish.

The supporting acute toxicity study to rainbow trout, carp, catfish and bluegill was performed according to the method reported by Bathe et al. (1974). Various concentrations of FeNa-EDDHA were prepared by adding the test material dissolved in aqua dest. (stock solution), at the appropriate amount ensuring that the total volume in each vessel remained the same. The LC50 for a 96 hour exposure was greater than 100 mg/L for Rainbow trout, Carp, Catfish and Bluegill.

Based on the structural similarity, the similar physico-chemical properties and the similar toxicity to aquatic invertebrates and algae between FeNa-EDDHA and MEAHA-Fe, identical conclusions are drawn for the read-across target MEAHA-Fe.