Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

The EC50 (3 h) was determined to be 28.8 mg a.s./L in an OECD test 209 (GLP, reliability 1). The EC10 (3h) was 10.9 mg a.s./L.

Key value for chemical safety assessment

EC50 for microorganisms:
28.8 mg/L
EC10 or NOEC for microorganisms:
10.9 mg/L

Additional information

Two studies are available on the toxicity to micro-organisms. The study by Kay (1959) was assigned reliability 3 because of missing data, lack of information on test material identity, composition, phys chem properties, analytics, culture of micro-organisms. The key study was performed with the substance as registered (quatenary ammonium compounds, C12 -18 -alkylbis(hydroxyethyl)methyl, chloride) according to OECD TG 209 using activated sludge, and in compliance with the OECD principles of Good Laboratory Practice (reliability 1).

The toxicity of quatenary ammonium compounds, C12 -18 -alkylbis(hydroxyethyl)methyl, chloride to activated sludge was determined by measuring the inhibition of the respiration at a contact time of 3 hours. The EC50 is 29 mg/L with 95% confidence limits of 15 and 56 mg/L. The corresponding EC10 is 11 mg/L. Based on active substance the EC50 = 28.8 mg/L with 95% confidence limits of 14.9 and 55.7 mg/L. The EC10 is 10.9 mg a.s./L