Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 916-533-5 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test material is considered to be readily biodegradable, with a mean degradation of 84.4 % after 28 days, OECD 301D, EPA OPPTS 835.3110, EU Method C.4-E, Sipos (2013a).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
In the key study (Sipos, 2013a) the test material was investigated for its ready biodegradability in a closed bottle test over a period of 28 days. The study was performed under GLP conditions and in accordance with the standardised guidelines OECD 301 D, EU Method C.4 E and EPA OPPTS 835.3110.
The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. Samples were incubated for 28 days in the dark with the test material at 3.1 mg/L. The test system was a microbial inoculum collected from the sewage plant for domestic sewage. The sludge was washed by centrifugation filtered through cotton wool and aerated until use. Based on the determined dry weight content, the washed sludge suspension contained 4 g dry material per litre.
Sodium benzoate was used as a procedural control, tested simultaneously under the same test conditions. Results confirmed the suitability of the activated sludge inoculums.
Under the test conditions the percentage biodegradation of test material reached a mean of 84.4 % after 28 days based on the measured COD of the test material. According to the test guidelines the pass level for ready biodegradability, 60 % of COD and this level has to be reached in a 10-day window within the 28-day period of the test. However taking into account that the test material was sufficiently degraded to a mean of 68.4 % after 14 days and according to the test guideline the value obtained in a 14-day window is acceptable in the used method, therefore the test material can be considered to be ready biodegradable.
In the toxicity control containing both, the test material and the reference substance (sodium benzoate), a mean of 46.7 % biodegradation was noted within 14 days and 54.7 % biodegradation after 28 days of incubation. According to the test guidelines the test material can be assumed to be not inhibitory at the applied concentration level of 3.1 mg/L on the activated sludge microorganisms because degradation was >25 % within 14 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
