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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure is in accordance with national standard methods with acceptable restrictions.

Data source

Reference
Reference Type:
publication
Title:
Aquatic environmental safety assessment for a nonphosphate detergent builder.
Author:
Maki AW and Macek KJ
Year:
1978
Bibliographic source:
Environ. Sci. Technol. 12, 573-580

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Methods for Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians. EPA 660/3-75/009 (1975)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Type A Zeolite
- Related CAS number: 1318-02-1
- Surface modified with Ca2+, Mg2+ and K+ ions. Analysis by atomic adsorption indicated that 90% existed in the Ca2+-modified form with a total of 10% as Mg2+, Na+ and K+ salts.

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Continuous gentle stirring was achieved by a motor-driven stirrer providing a water current through perforated tubing holes to keep the test substance in suspension.

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: fathead minnows
- Source: acquired from a hatchery in Arkansas
- Age at study initiation (mean and range, SD):
- Length at study initiation (mean): 53 mm
- Weight at study initiation (mean): 1.3 g

ACCLIMATION
- Acclimated to laboratory test conditions for a minimum of 30 days prior to testingcclimation period:

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
35 mg/l CaCO3
Test temperature:
21 +- 1°C
pH:
7.1
Dissolved oxygen:
oxygen content above 6 mg/l.
Nominal and measured concentrations:
Concentrations up to 680 mg/L were tested (not further specified).
Details on test conditions:
TEST SYSTEM
- Test vessel: 19.6 L glass container, kept in water baths
- No. of organisms per vessel: 10, fish per concentration were added to each test container within 30 min. after the compound was added
- No. of vessels per concentration (replicates): no data
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): no data
- No feeding during the test and 48 h prior to testing.

TEST MEDIUM / WATER PARAMETERS
- Dilution water was reconstituted deionized water, a control with dilution water only was maintained.

OTHER TEST CONDITIONS
- Not aerated

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Mortality

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
> 680 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Any other information on results incl. tables

Test organisms exhibited no mortality up to 680 mg/L.

Applicant's summary and conclusion