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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2002-11-25
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Sächsisches Staatsministerium für Umwelt und Landwirtschaft, Sachsen, Germany

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Zeolite A, Ca++-form: Vegobond 5A Powder
- Related CAS number (according to inventory issues): 1327-36-2
- Related CAS number: 1318-02-1
- Physical state: white powder
- Analytical purity: 80.65%
- Lot/batch No.: 1103
- Storage condition of test material: room temperature, dark and dry
- Surface modified with Ca

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
agricultural soil from site Gerichshain, stored >1 year before use in the test, no pesticides and fertilisers applied for at least 5 years
Soil characteristics: sandy loam, pH 6.0, 0.8% organic carbon content, 1.4% humus content, 36.7% water holding capacity, particle size: 9.7% clay, 49.7% silt, 40.6% sand, 35.7% washed out particles.

Test organisms

Species:
Raphanus sativus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Seeds (Raphanus sativus Rodos, seed germination rate 92%) were purchased from Dehner Gartencenter, D-04425 Taucha.

Study design

Test type:
other: OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
18-30 °C
pH:
6.0
Moisture:
relative humidity: 15-37%
Nominal and measured concentrations:
Following concentrations were tested: 0, 15, 30, 60, 120 and 240 g/kg d.w.

Results and discussion

Effect concentrationsopen allclose all
Species:
Raphanus sativus
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
> 240 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Raphanus sativus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
240 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot fresh weight
Species:
Raphanus sativus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
60 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: plant height

Any other information on results incl. tables

Emergence in the control group was 100%, no phytotoxic effects visible.
LC50/EC50 was >240 g/kg d.w. for all parameters.
NOEC for shoot fresh weight was 240 g/kg d.w. A statistically significant increase in shoot fresh weight was observed at 60, 120 and 240 g/kg, the resulting NOEC for shoot fresh weight increase was 30 g/kg d.w.
NOEC for plant height was 60 g/kg.
No visual signs of phototoxicity (chlorosis, necrosis) were observed at any dose level.
The results are based on nominal concentrations of the test substance.

Applicant's summary and conclusion