Registration Dossier

Administrative data

Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure is in accordance with national standard methods with acceptable restrictions (no GLP).

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Aquatic environmental safety assessment for a nonphosphate detergent builder.
Author:
Maki AW and Macek KJ
Year:
1978
Bibliographic source:
Environ. Sci. Technol. 12, 573-580.
Reference Type:
publication
Title:
Correlations between Daphnia magna and fathead minnow (Pimephales promelas) chronic toxicity values for several classes of test substances.
Author:
Maki AW
Year:
1979
Bibliographic source:
J. Fish. Res. Board Can. 36, 411-421.

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: U.S. Environmental Protection Agency, Proposed recommended bioassay procedures for egg and fry stages of freshwater fish, Duluth, Minn., 1972
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Type A zeolite
- Related CAS number: 1318-02-1
- Type A zeolite was surface modified: Analysis by atomic adsorption indicated that 90% existed in the Ca2+-modified form with a total of 10% as Mg2+, Na+ and K+ salts.

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
- Analysis by the silicate method (APHA 1977)

Test organisms

Test organisms (species):
Pimephales promelas

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 d

Test conditions

Hardness:
36 mg/L CaCO3
Test temperature:
no data
pH:
pH 7.6 +/- 0.3
Dissolved oxygen:
> 86% saturation
Nominal and measured concentrations:
0, 6.2, 12.3, 22.6 and 86.7 mg/L (mean measured concentrations).

Results and discussion

Effect concentrations
Duration:
30 d
Dose descriptor:
NOEC
Effect conc.:
> 86.7 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: hatch, survival, mean total length, average weight

Any other information on results incl. tables

The results indicate no adverse effects on hatchability of eggs and survival, mean total length, and average weight of fry at 30 days post hatch during all exposures to zeolite A.
The concentrations of test materials in suspension varied considerably during the exposure period, reflecting the difficulty of maintaining uniform suspensions of these particulates. Highest zeolite concentrations varied from 33 to 182 mg/L with a mean of 87 mg/L. Thus, the observed NOEC was determined to be greater than this value.

Applicant's summary and conclusion