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EC number: 201-328-9
CAS number: 81-14-1
In an in vitro skin irritation assay in a human epidermal model EpiDerm
(17-767), PBS-moistened reconstructed human epidermis tissue was exposed
to 25 mg of musk ketone (99.87%) for 60±1 minutes (25 minutes at room
temperature and 35 minutes at 37±1°C). PBS was used for the negative
control and 5% SDS was used for the positive control. After removal of
the test substance, tissues were post-incubated for approximately 42
hours. Three hours incubation with MTT and a 135 minute isopropyl
alcohol extraction period followed. The OD570 of isopropyl alcohol
extracts was measured on a spectrophotometer. Relative cell viability
was calculated for each tissue as % of the mean viability of the
negative control tissues.
The colour of the test substance did not interfere with the endpoint.
The test substance is not directly MTT reducing. The average viability
of tissues treated by the test substance musk ketone was 102.4 % ±1.2%
of negative control average value i.e. viability was > 50 %. The average
viability of tissues treated by the positive control (5% SDS) was 2.4 %
±0.2% of negative control average value. According to these results, the
test substance is not irritating.
This in vitro skin irritation study in the human epidermal model EpiDerm
is acceptable and satisfies the guideline requirement for an OECD 439
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